Phototoxicity frequently occurs during live fluorescence microscopy, and its consequences are often underestimated. Damage to cellular macromolecules upon excitation light illumination can impair sample physiology, and even lead to sample death. In this review, we explain how phototoxicity influences live samples, and we highlight that, besides the obvious effects of phototoxicity, there are often subtler consequences of illumination that are imperceptible when only the morphology of samples is examined. Such less apparent manifestations of phototoxicity are equally problematic, and (...) can change the conclusions drawn from an experiment. Thus, limiting phototoxicity is a prerequisite for obtaining reproducible quantitative data on biological processes. We present strategies to reduce phototoxicity, e.g. limiting the illumination to the focal plane and suggest controls for phototoxicity effects. Overall, we argue that phototoxicity needs increased attention from researchers when designing experiments, and when evaluating research findings. (shrink)

Microscopy allows us to observe objects we cannot see with our eyes alone. With a light microscope, we can distinguish objects at the scale of the wavelengths of visible light just under a micrometer. Around 1870 Ernst Abbe, who laid the foundation of modern optics, suggested that the resolution of a microscope would improve by using some yet-unknown radiation with shorter wavelengths than visible light, that is, below 390 nanometers (1 nm = 10−9 m). Electrons can (...) have wavelengths near 1 picometer (1 pm = 10−12 m) and should therefore allow atoms to be distinguished since they are typically at least a few hundred pm apart. In this oversimplified view, further decreasing the wavelength of the radiation should allow us to increase the resolution of electron microscopy even more. However, this can only be done by increasing the energy of the radiation, which would ultimately destroy the samples. Other factors also affect the resolution of electron microscopes, among them non-ideal imaging properties of electromagnetic lenses, which result in false images. It took more than half a century to understand and control these aberrations and separate objects less than 1 Ångstrom (1 Å = 10−10 m) apart. (shrink)

Light sheet microscopy is an easy to implement and extremely powerful alternative to established fluorescence imaging techniques such as laser scanning confocal, multi‐photon and spinning disk microscopy. By illuminating the sample only with a thin slice of light, photo‐bleaching is reduced to a minimum, making light sheet microscopy ideal for non‐destructive imaging of fragile samples over extended periods of time. Millimeter‐sized samples can be imaged rapidly with high resolution and high depth penetration. A large (...) variety of instruments have been developed and optimized for a number of different samples: Bessel beams form thin light sheets for single cells, and selective plane illumination microscopy (SPIM) offers multi‐view acquisition to image entire embryos with isotropic resolution. This review explains how light sheet microscopy involves a conceptually new microscope design and how it changes modern imaging in biology. (shrink)

The introduction of electron microscopy profoundly altered biomedical research, providing a tool for a more detailed but at the same time a spatially and temporally more restricted visual analysis. Examining the case study of Golgi apparatus research in the 1950s and 1960s, it will be shown how microscopists handled these challenges, and how these confrontations modified the general concept of cellular organization. This will also shed light on the artifact debate and on the question of scientific realism in (...) the field of microscopy. (shrink)

Gap junction intercellular communication channels permit the exchange of small regulatory molecules and ions between neighbouring cells and coordinate cellular activity in diverse tissue and organ systems. These channels have short half‐lives and complex assembly and degradation pathways. Much of the recent work elucidating gap junction biogenesis has featured the use of connexins (Cx), the constituent proteins of gap junctions, tagged with reporter proteins such as Green Fluorescent Protein (GFP) and has illuminated the dynamics of channel assembly in live cells (...) by high‐resolution time‐lapse microscopy. With some studies, however, there are potential short‐comings associated with the GFP chimeric protein technologies. A recent report by Gaietta et al., has highlighted the use of recombinant proteins with tetracysteine tags attached to the carboxyl terminus of Cx43, which differentially labels ‘old’ and ‘new’ connexins thus opening up new avenues for studying temporal and spatial localisation of proteins and in situ trafficking events.1 BioEssays 24:876–880, 2002. © 2002 Wiley Periodicals, Inc. (shrink)

The laser scanning confocal microscope (LSCM)LSCM: laser scanning confocal microscope; FISH: fluorescence in situ hybridisation; DiO6: 3,3′‐dihexyloxacarbocyanine iodide; NBD‐ceramide: 6‐((N‐(7‐nitrobenz‐2‐oxa‐1,3‐diazol‐4‐yl)amino)‐caproyl)sphingosine; DiO: 3,3′‐dioctadecyloxacarbocyanine perchlorate; DiI: 1,1′‐dioctadecyl‐3,3,3′,3′‐tetramethyl‐indocarbocyanine perchlorate; CCD: charge‐coupled device; DIC: differential interference contrast; FURA2: (‐(2‐(5‐carboxyoxazol‐2‐yl)‐6‐aminobenzofuran‐5‐oxy)‐2‐)2′‐amino‐5′‐methylphenoxy)‐ethane‐N,N,N′,N′‐ tetraacetic acid, sodium salt);BCECF: 2′,7′‐bis‐(carboxyethyl)‐5‐(and‐6‐)‐carboxyfluorescein;fluo‐3: 1‐(2‐amino‐5‐(2,7‐dichloro‐6‐hydroxy‐3‐oxo‐3H‐xanthen‐9‐yl)‐2‐(2′amino‐5′‐methylphenoxy)‐ethane‐N,N, N′,N′,‐tetraacetic acid, ammonium salt; DAPI: 4′,6‐diamidino‐2‐phenylindole, dihydrochloride; PET: positron emission tomogrophy; CT: computer‐assisted tomogrophy; CiD: cubitus interruptus dominus; MRC: Medical Research Council; TOTO‐1: benzothiazolium‐4‐quinolinium dimer; YOYO‐1: benzoxazolium‐4‐quinolinium dimer; ex.: excitation wavelength; em.: emission wavelength. is now established as an invaluable tool in (...) developmental biology for improved light microscope imaging of fluorescently labelled eggs, embryos and developing tissues. The universal application of the LSCM in biomedical research has stimulated improvements to the microscopes themselves and the synthesis of novel probes for imaging biological structures and physiological processes. Moreover the ability of the LSCM to produce an optical series in perfect register has made computer 3‐D reconstruction and analysis of light microscope images a practical option. (shrink)

Fluorescence microscopy is a powerful tool used in scientific and medical research, but it is inextricably linked to phototoxicity. Neglecting phototoxicity can lead to erroneous or inconclusive results. Recently, several reports have addressed this issue, but it is still underestimated by many researchers, even though it can lead to cell death. Phototoxicity can be reduced by appropriate microscopic techniques and carefully designed experiments. This review focuses on recent strategies to reduce phototoxicity in microscopic imaging of living cells and tissues. (...) We describe digital image processing and new hardware solutions. We point out new modifications of microscopy methods and hope that this review will interest microscopy hardware engineers. Our aim is to underscore the challenges and potential solutions integral to the design of microscopy systems. Simultaneously, we intend to engage biologists, offering insight into the latest technological advancements in imaging that can enhance their understanding and practice. (shrink)

Soft X‐ray tomography (SXT) is an imaging technique capable of characterizing and quantifying the structural phenotype of cells. In particular, SXT is used to visualize the internal architecture of fully hydrated, intact eukaryotic and prokaryotic cells at high spatial resolution (50 nm or better). Image contrast in SXT is derived from the biochemical composition of the cell, and obtained without the need to use potentially damaging contrast‐enhancing agents, such as heavy metals. The cells are simply cryopreserved prior to imaging, and (...) are therefore imaged in a near‐native state. As a complement to structural imaging by SXT, the same specimen can now be imaged by correlated cryo‐light microscopy. By combining data from these two modalities specific molecules can be localized directly within the framework of a high‐resolution, three‐dimensional reconstruction of the cell. This combination of data types allows sophisticated analyses to be carried out on the impact of environmental and/or genetic factors on cell phenotypes. (shrink)

The exocyst is a conserved octameric complex that physically tethers a vesicle to the plasma membrane, prior to membrane fusion. It is important not only for secretion and membrane delivery but also, in mammalian cells, for cytokinesis, ciliogenesis, autophagy, tumorigenesis, and host defense. The combination of genome editing and advanced light microscopy of exocyst subunits in living cells has recently shown the complex to be much more dynamic than previously appreciated, and exposed how little we still know about (...) its function and regulation. (shrink)

The paper describes the first attempts of biological electron microphotography. It starts with a description of the early use of electron microscopy in biology, showing that electron microscopy was used as an extension of former light microscopical studies. Thus, the pictures produced by electron microscopy are interpreted as describing the micro-texture of those structures already seen in light microscopy. That was done irrespective from the specific problems of tissue preparation for electron microscopy. The (...) use of photography in electron microscopy is discussed in more detail. It is shown that in electron microscopy, the microphotography provides the data base for the observing scientist, not the preparation itself which is usually destroyed or damaged during observation in the electron microscope. Thus, biological electron microscopy can be described to be a real image science. (shrink)

The nuclear export of mRNA through the nuclear pore complex (NPC) is a process required for the healthy functioning of human cells, making it a critical area of research. However, the geometries of mRNA and the NPC are well below the diffraction limit of light microscopy, thereby presenting significant challenges in evaluating the discrete interactions and dynamics involved in mRNA nuclear export through the native NPC. Recent advances in biotechnology and single‐molecule super‐resolution light microscopy have enabled (...) researchers to gain granular insight into the specific contributions made by discrete nucleoporins in the nuclear basket of the NPC to the export of mRNA. Specifically, by expanding upon the docking step facilitated by the protein TPR in the nuclear basket as well as identifying NUP153 as being the primary nuclear basket protein initiating export through the central channel of the NPC. (shrink)

The essay introduces how Paul A. Weiss (1898-1989) analyzed his data on neuronal outgrowth and axonal transport, supported by constriction experiments of thousands of living mature nerve fibers. At the University of Chicago his group measured the steady proximo-distal flow of nerve fibers. To visualize the data he used tissue culturing, light microscopy, radioactive tracers, time-lapse motion pictures and electronmicroscopy. The work resulted in the discovery of fasciculation of outgrowing nerves and a computation of the rate of axonal (...) transport, published in a classical article in 1948. He stated that the outgrowth of nerve fibers occurs from nerve centers in their nucleated cell bodies by fasciculation and protein synthesis. However, at that time one suspected the published microphotograph to be an idealized image and, therefore, did not accept the analysis, or the 'new' knowledge. In the mid 1960s the improved technique of autoradiography confirmed in an indirect way Weiss' data and analysis of axonal transport. The objective here is to show (1) how Weiss employed the visibility of micrographs and drawings to corroborate his observations and analysis on axonal dynamics, and (2) to offer some tentative suggestions why his colleagues did not accept the 'neuro-images' as an evidence of new knowledge. (shrink)

Embryos and microscopes share a long, remarkable history and biologists have always been intrigued to watch how embryos develop under the microscope. Here we discuss the advances in microscopy which have greatly influenced our current understanding of embryogenesis. We highlight the evolution of microscopes and the optical technologies that have been instrumental in studying various developmental processes. These imaging modalities provide mechanistic insights into the dynamic cellular and molecular events which drive lineage commitment and morphogenetic changes in the developing (...) embryo. We begin the journey with a brief history of microscopy to study embryos. First, we review the principles and optics of light, fluorescence, confocal, and electron microscopy which have been key techniques for imaging cellular and molecular events during embryonic development. Next, we discuss recent key imaging modalities such as light‐sheet microscopy, which are suitable for whole embryo imaging. Further, we highlight imaging techniques like multiphoton and super resolution microscopy for beyond light diffraction limit, high resolution imaging. Lastly, we review some of the scattering‐based imaging methods and techniques used for imaging human embryos. (shrink)

This series of images forms part of the ongoing project Soft Ground, Hard Light, which speculates on a multiscalar spatial ontology of photography emanating from the Arditurri silver mine complex in the Basque Country. Evoking the mountainous massif around Arditurri where silver ore and galena have been extracted since Roman presence in the area, the depicted topographies are, in fact, data transcriptions from atomic force microscopy (AFM) probing the analogue film stock of an early 1930s experiment in 3D (...) cinema, Cine en Relieve, by the Basque cinematographer Teófilo Mingueza. In contrast to optical or electron microscopy, AFM uses a scanning probe to physically touch the specimen, recording its surface undulations at the atomic scale of a nanometre, or a billionth of a metre. As a haptic operation based on direct contact between instrument and sample, between apparatus and referent, AFM is literally a contact print, a ‘blind’ scan that senses not the visual content of the recorded image but ‘feels’ its underlying material substrate of silver nanoparticles within the 35 mm film emulsion. The resulting image assemblies visualize a topography resulting from the intra-action between the silver nanoparticles and the scanning probe as much as its transcription through the particular parameters afforded by the AFM analysis software. Hard Light, Soft Ground was initiated during an artist’s residency at Tabakalera Centre for Contemporary Culture, Donostia-San Sebastián, and is currently in production as part of the wider research project Esper Syndrome: Archaeotopologies of the Image at the Royal College of Art, funded by the AHRC through the London Arts & Humanities Partnership. With additional thanks to the Basque Film Archive and Gustavo Ariel Schwartz at the Materials Physics Centre, Donostia-San Sebastián. (shrink)

Several models of macromolecular arrangements in eukaryotic chromosomes have been proposed during the past fifteen years. Many of the models are consistent with physical and chemical data on the molecular components of chromosomes, and a few have the appearance of meeting the requirements for cytological organization in chromosomes. However, one of the most frustrating problems in developing a working model is to provide a scheme that fits genetic function while satisfying the structural parameters. This has not yet been achieved.Although emphasis (...) in this review has been placed on uninemic and polynemic models, alternatives, such as a bineme, for example, remain. It is clear, moreover, that the issue can be resolved only through continued efforts to make direct observations of chromosomes with light and electron microscopy coupled with the additional tools ofX-ray analysis and analytical biochemistry. A recent analysis byWray andStubblefield has led to a rather innovative model of the chromosome, and exemplifies the kind of approach needed to clarify the phenomenon. Furthermore, analyses of meiotic chromosomes may provide valuable insight for relating organization to genetic function . Of particular interest are mutation events as related to subchromatid organization, and the reorganization of chromosomal fibrils during early meiotic stages. At present, and as a generalization, the evidence points more strongly toward at least a binemic arrangement of chromosomal subunits than toward a uninemic one. (shrink)

Transport through the nuclear pore complex (NPC), a keystone of the eukaryotic building plan, is known to involve a large channel and an abundance of phenylalanine–glycine (FG) protein domains serving as binding sites for soluble nuclear transport receptors and their cargo complexes. However, the conformation of the FG domains in vivo, their arrangement in relation to the transport channel and their function(s) in transport are still vividly debated. Here, we revisit a number of representative transport models—specifically Brownian affinity gating, selective (...) phase gating, reversible FG domain collapse, and reduction of dimensionality (ROD)—in the light of new data obtained by optical single transporter recording, optical superresolution microscopy, artificial nanopores, and many other techniques. The analysis suggests that a properly adapted, simplified version of the ROD model accounts well for the available data. This has implications for nucleocytoplasmic transport in general. (shrink)

Segmentation of the medical image plays a significant role when it comes to diagnosis using computer aided system. This article focuses on the human corneal endothelium’s health, which is one of the filed research interests, especially in the human cornea. Various pathological environments fasten the extermination of the endothelial cells, which in turn decreases the cell density in an abnormal manner. Dead cells worsen the hexagonal design. The mutilated endothelial cells can no longer revive back and that gives room for (...) neighbouring cells to migrate and expand so that they can fill in the space. The latter results in cell elongation that is unpredictable as well as increase in size and thinning. Cell density and shape are therefore considered major parameters when it comes to explaining the health condition attributed to corneal endothelium. In this study, medical feature extraction was obtained depending on the segmentation of the endothelial cell boundary, and the task of segmentation of such objects especially the thin, transparent, and unclear cell boundary is considered challenging due to the nature of the image capture during endothelium layer examination by ophthalmologists using confocal or specular microscopy. The resulting image suffers from various issues that affect the quality of the image. Low quality is due to non-uniformity of illumination and the presence of a lot of noise and artefacts resulting from high amounts of distortion, and most of these limitations are present because of the nature of the imaging modality. Usually, images contain certain kind of noise and also continuous shadow. Furthermore, the cells are separated by poor border, thereby leading to great difficulty in the segmentation of the images. The irregular shape of cell and also the contrast of such images seem to be low as they possess blurry boundaries with diverse objects existing in addition to the lack of homogeneity. The main aim of the study is to propose and develop a totally automatic, robust, and real-time model for the segmentation of endothelial cells of the human cornea obtained by in vivo microscopy and computation of different clinical features of endothelial cells. To achieve the aim of this study a new scheme of image enhancement was proposed such as the Contrast-Limited Adaptive Histogram Equalisation technique to enhance contrast. After that, a new image denoising technique called Wavelet Transform Filter and Butterworth Bandpass for Segmentation is used. Subsequently, brightness level correction is applied by using the moving average filter and the CLAHE to reduce the effects of the non-uniform image lighting produced as a result of the previous step. The main aim of this article is the segmentation of endothelial cells, which involves precise detection of the endothelial contours. So a new segmentation model was proposed such that the shape of the cells will be extracted, and the contours were highlighted. This stage is followed by clinical feature extraction and uses the features for diagnosis. In this stage, several relevant clinical features such as pleomorphism mean cell perimeter, mean cell density, mean cell area, and polymegathism are extracted. The role of these clinical features is crucial for the early detection of corneal pathologies as well as the evaluation of the health of the corneal endothelium layer. The findings of this study were promising. (shrink)

Synaptic ribbons, the organelles identified in electron micrographs of the sensory synapses involved in vision, hearing, and balance, have long been hypothesized to play an important role in regulating presynaptic function because they associate with synaptic vesicles at the active zone. Their physiology and molecular composition have, however, remained largely unknown. Recently, a series of elegant studies spurred by technical innovation have finally begun to shed light on the ultrastructure and function of ribbon synapses. Electrical capacitance measurements have provided (...) sub‐millisecond resolution of exocytosis, evanescent‐wave microscopy has filmed the fusion of single 30 nm synaptic vesicles, electron tomography has revealed the 3D architecture of the synapse, and molecular cloning has begun to identify the proteins that make up ribbons. These results are consistent with the ribbon serving as a vesicle “conveyor belt” to resupply the active zone, and with the suggestion that ribbon and conventional chemical synapses have much in common. BioEssays 23:831–840, 2001. © 2001 John Wiley & Sons, Inc. (shrink)

This paper explores the different identities adopted by connective tissue research at the University of Manchester during the second half of the 20th century. By looking at the long-term redefinition of a research programme, it sheds new light on the interactions between different and conflicting levels in the study of biomedicine, such as the local and the global, or the medical and the biological. It also addresses the gap in the literature between the first biomedical complexes after World War (...) II and the emergence of biotechnology. Connective tissue research in Manchester emerged as a field focused on new treatments for rheumatic diseases. During the 1950s and 60s, it absorbed a number of laboratory techniques from biology, namely cell culture and electron microscopy. The transformations in scientific policy during the late 70s and the migration of Manchester researchers to the US led them to adopt recombinant DNA methods, which were borrowed from human genetics. This resulted in the emergence of cell matrix biology, a new field which had one of its reference centres in Manchester. The Manchester story shows the potential of detailed and chronologically wide local studies of patterns of work to understand the mechanisms by which new biomedical tools and institutions interact with long-standing problems and existing affiliations. (shrink)

Nanotechnologies produce many different types of images but are characterized by the ones that allow us to ‘see the atoms’ despite the fact that objects at the nanoscale are smaller than the wavelength of light and hence are ‘invisible’. Images from scanning probe microscopy (SPM), like ‘The Beginning’, have played an emblematic role in the constitution of the field and are also more likely to be used in communication outside the scientific field. These images are made, selected, modified (...) and evaluated with respect to the information that they communicate. The ‘life cycle’ of these images implies the transduction of this information. Outside of the scientific field, however, they are no longer defined by this information, nor according to technical and scientific criteria, but by their aesthetic power, and they become associated with other images from other fields (art, advertisement, entertainment). This process creates a psycho-social ‘halo’ about ‘nano’ in the public perception, and raises ethical issues about nanotechnological communication via images. We will analyze the halo of the nano in this respect and propose a strategy for a reflexive ‘accommodation’ to the images at the nanoscale. (shrink)

Novel forms of matter, such as states made of gluons (glueballs), multiquark mesons or baryons and hybrid mesons are predicted by low energy QCD, for which several candidates have recently been identified. Searching for such exotic states of matter and studying their production and decay properties in detail has become a flourishing field at the experimental facilities now available or being built - e.g. BESIII in Beijing, BELLE II at SuperKEKB, GlueX at Jefferson Lab, PANDA at FAIR, J-PARC and in (...) the upgraded LHC experiments, in particular LHCb. A modern primer in the field is required so as to both revive and update the teaching of a new generation of researchers in the field of QCD. These lectures on hadron spectroscopy are intended for Master and PhD students and have been originally developed for a course delivered at the Stefan Meyer Institute of the Austrian Academy of Sciences. They are phenomenologically oriented and intended as complementary material for basic courses in particle and nuclear physics. The book describes the spectra of light and heavy mesons and baryons, and introduces the fundamental properties based on symmetries. Further, it derives multiplet structures, mixing angle, decay coupling constants, magnetic moments of baryons, and predictions for multiquark states and compares these with suitable experimental data. Basic methods of calculating decay angular distributions and determining masses and widths of resonances are also presented. The appendices provide students and newcomers to the field with the necessary background information, and include a set of problems and solutions. (shrink)

This chapter explores the ethical dimensions of diplomatic efforts to form a global agreement on climate change. It offers a brief historical background on the core multilateral climate negotiation body, the United Nations Framework Convention on Climate Change, and highlights some contentious moral elements of these negotiations. In particular, it explores the complex ways in which the principle of “common but differentiated responsibilities” has driven debates on how burdens for mitigation, adaptation, and finance should be distributed between developed and developing (...) countries. It then considers the transformation in these climate negotiations since 2009, including the move toward a bottom-up architecture as part from the Copenhagen Accord to the Paris Agreement. Finally, it assesses the current state of climate diplomacy in relation to broader diplomatic priorities, arguing that climate diplomacy must be elevated alongside other top-tier foreign policy issues today in order to eventually achieve some level of climate stability. (shrink)

Empathy is the combined ability to interpret the emotional states of others and experience resultant, related emotions. The relation between prefrontal electroencephalographic asymmetry and emotion in children is well known. The association between positive emotion (assessed via parent report), empathy (measured via observation), and second-by-second brain electrical activity (recorded during a pleasurable task) was investigated using a sample of one hundred twenty-eight 6- to 10-year-old children. Contentment related to increasing left frontopolar activation (p < .05). Empathic concern and positive empathy (...) related to increasing right frontopolar activation (ps < .05). A second form of positive empathy related to increasing left dorsolateral activation (p < .05). This suggests that positive affect and (negative and positive) empathy both relate to changes in prefrontal activity during a pleasurable task. (shrink)

Discussion of ecological restoration in environmental ethics has tended to center on issues about the nature and character of the values that may or may not be produced by restored landscapes. In this paper we shift the philosophical discussion to another set of issues: the social and political context in which restorations are performed. We offer first an evaluation of the political issues in the practice of restoration in general and second an assessment of the political context into which restoration (...) is moving. The former focuses on the inherent participatory capacity at the heart of restoration; the latter is concerned with the commodified (primarily in the United States) and nationalized (primarily in Canada) uses to which restoration is being put. By comparing these two areas of inquiry we provide a foundation for a critical assessment of the politics of restoration based on the politics in restoration. (shrink)

Essays showing how environmental philosophy can have an impact on the world by integrating abstract reasoning with actual environmental practice.

Institutional corruption is a normative concept of growing importance that embodies the systemic dependencies and informal practices that distort an institution’s societal mission. An extensive range of studies and lawsuits already documents strategies by which pharmaceutical companies hide, ignore, or misrepresent evidence about new drugs; distort the medical literature; and misrepresent products to prescribing physicians. We focus on the consequences for patients: millions of adverse reactions. After defining institutional corruption, we focus on evidence that it lies behind the epidemic of (...) harms and the paucity of benefits. (shrink)

As of January 1, 2008, over 98,000 people are waiting for organ transplants in the United States of America. Of those, nearly 75,000 are waiting for a kidney. In this calendar year, fewer than 15,000 will receive a kidney transplant from a deceased donor. The average waiting time for a deceased donor kidney now exceeds five years in virtually all metropolitan areas. Sadly, nearly as many people die waiting as there are deceased donors each year, despite monumental efforts by the (...) entire transplant community to increase both the number of organ donors and the number of organs recovered from each donor. The imbalance between demand and supply has led to considerable efforts to expand the criteria for what is considered an acceptable organ donor by the Organ Procurement and Transplant Network, thereby hoping somewhat to assuage the shortfall of donor organs. So-called Expanded Criteria Donors may be older than 50, have history of hypertension, or have died from intracerebral hemorrhage and/or have impaired renal function. ECDs now make up nearly 40% of the donor population. (shrink)

Light and Kennison found that bias effects in the forced-choice perceptual identification of words occurred only in a subset of participants, those who claimed on a strategy questionnaire to be deliberately guessing words they had studied previously. McKoon and Ratcliff raised a number of objections to the proposal that bias effects are due to guessing strategies, citing difficulties in our statistical treatment of data, our use of subjective reports to classify participants, and our approach to the general problem of (...) separating implicit from explicit influences on performance. This article responds to these objections. (shrink)

Selectional preferences have a long history in both generative and computational linguistics. However, since the publication of Resnik's dissertation in 1993, a new approach has surfaced in the computational linguistics community. This new line of research combines knowledge represented in a pre‐defined semantic class hierarchy with statistical tools including information theory, statistical modeling, and Bayesian inference. These tools are used to learn selectional preferences from examples in a corpus. Instead of simple sets of semantic classes, selectional preferences are viewed as (...) probability distributions over various entities. We survey research that extends Resnik's initial work, discuss the strengths and weaknesses of each approach, and show how they together form a cohesive line of research. (shrink)

Schizophrenia patients exhibit impairments across multiple clinical, cognitive, and functional domains. A fundamental abnormality of the timing and/or efficiency of neural processes across disparate brain regions (i.e., cortico-cortical communications) may underlie many of the deficits in schizophrenia. Because gamma synchrony is temporally correlated with many cognitive processes, probing patterns of gamma activation may shed light on the functional integrity of neural circuits in schizophrenia and related disorders.

It is an old wag among environmentalists that humans have become disconnected from nature. The culprits for this conundrum are various. If it is not our addiction to technological enticements then it is our life in big cities which alienate us from our “earthen elements.” The presumed result of this disconnection is that we do not respect the land anymore and turn a blind eye to the environmental consequences of our collective acts of consumption and pollution. Various bits of evidence (...) are produced to prove this point – mostly anecdotal – such as the claim that many city-dwellers, when asked where their food comes from, will respond blankly, “from a grocery store.” What is the curative for this ailment? Surprisingly, it is not that we should send urbanites out to the factory farms, county-sized feed lots, or flavor factories in New Jersey, which actually put most of the food on the shelves of neighborhood markets. It is instead usually suggested that we should send people to wilderness areas, that we should become more connected with nature in the raw, as it were. E. O. Wilson’s “biophilia” hypothesis is a good case in point. Defending a sociobiological account of why humans are innately attracted to living things, Wilson suggests that this connection is best realized in the residual attachment of humans to wild nature. This grounds a claim that the most important task at hand is to focus on “the central questions of human origins in the wild environment” (Wilson 1992, 351). It is probably unfair to suggest that Wilson thinks that we should all go to the wilderness in order to be better connected with nature, and implicitly, to then.. (shrink)

T. C. Boyle’s A Friend of the Earth (2001), tells the story of Tyrone Tierwater, a one time monkeywrencher and environmental avenger for “E. F.!” (Earth Forever!) who we first meet in 2025 in his mid-seventies. Tierwater is now working for a character based on Michael Jackson, who in his semi-retirement has employed the elder eco-warrior to help save some of the last remnants of a few dying species – warthogs, peccaries, hyenas, jackals, lions and what is likely the last (...) Patagoninan fox. The not too distant environmental future painted by Boyle is a disaster. Global warming has finally caught up to us with a vengeance and even the secure shores of the U.S. are wracked by unmitigated cycles of flooding and drought seriously degrading most semblances of life as we know it. To be sure, though, people, and some versions of progress, go on. While most affordable food and drink is limited to some combination of catfish and sake (very little else having survived decades of disastrous weather and a series of crop blights), and there are constant threats of new strains of life-threatening and highly contagious diseases, suburban development continues and new humans come into existence with the promise, at least in the developed world, of longer life spans. But Boyle does not give us anything like the overly optimistic views expressed by some conservative columnists who dismiss the need for global climate treaties; this is not an environmental future that is only felt with difficulty in the underdeveloped south requiring simpler economic readjustments for Americans without a substantial shift in lifestyle. The world.. (shrink)

The collection of papers that comprise this thesis explore three sets of questions important to environmental philosophy, broadly construed. All three topics are explored through the theoretical device of environmental pragmatism, the argument that philosophical disagreements on environmental questions can sometimes be set aside in order to achieve compatible strategies to work toward improving environmental conditions. As part of this strategy, pragmatists also call for the abandonment of the existing prejudices of environmental philosophy, in particular nonanthropocentrism and commitments to moral (...) monism. Part I of the thesis looks at two sets of debates in environmental philosophy: the social ecology-deep ecology divide in political ecology, and the debate between monists and pluralists in environmental ethics. Both debates are used as a vehicle to advance the pragmatist position, as well as demonstrate the connection between this thesis and other attempts to articulate a workable form of pluralism in environmental philosophy. Next, the first half of Part II uses the pragmatist premise to launch applied investigations of two environmental questions: the privatization of environmental regulations, and the political appropriation of restoration ecology. The second half of Part II explores questions concerning urban space and political identity, which come to the center of environmental philosophy following a pragmatist assault on its existing prejudices. Finally, Part III continues the themes of the last half of Part II to look at issues concerning technology and built space, topics which have traditionally been ignored by environmental philosophers. These final chapters present a set of issues concerning space and place which must be integral to an environmental philosophy which has been tempered by pragmatic concerns. (shrink)

Despite the intransigent nature of many of the problems discussed, the contributors to this volume demonstrate the possibilities for developing a viable alternative politics.

In recent centuries Christians of various denominations have endorsed many different political philosophies that they see as being truly biblical in their approach. Over this time there has been an increasing hostility, by some Christians, towards free markets and political philosophies that hold human liberty as the highest goal such as libertarianism and classical liberalism. This criticism is unwarranted and misplaced as libertarianism and free markets are not only compatible with Christianity, they are also the most biblically sound of all (...) economics systems and political philosophies endorsed by Christians today. Therefore, this paper will argue that Christians of all denominations should endorse free markets and libertarianism if they wish to create a world that follows biblical principles and the teachings of Jesus. (shrink)

Philosophers and geographers have converged on the topic of public space, fascinated and in many ways alarmed by fundamental changes in the way post-industrial societies produce space for public use, and in the way citizens of these same societies perceive and constitute themselves as a public. This volume advances this inquiry, making extensive use of political and social theory, while drawing intimate connections between political principles, social processes, and the commonplaces of our everyday environments.