The DNase I hypersensitive sites (DHSs) of chromatin constitute one of the best landmarks of eukaryotic genes that are poised and/or activated for transcription. For over 35 years, the high‐mobility group nucleosome‐binding chromosomal proteins HMGN1 and HMGN2 have been shown to play a role in the establishment of these chromatin‐accessible domains at transcriptional regulatory elements, namely promoters and enhancers. The critical presence of HMGNs at enhancers, as highlighted by a recent publication, suggests a role for them in (...) the structural and functional fine‐tuning of the DHSs in vertebrates. As we review here, while preferentially out‐competing histone H1 binding and invading neighbor nucleosomes, HMGNs may also modulate histone H3 at serine 10 (H3S10ph), which plays an important role in enhancer function and transcriptional initiation. (shrink)

The DNA in a eukaryotic nucleus is packaged into a nucleosome array, punctuated by variations in the regular pattern. The local chromatin structure of inducible genes appears to fall into two categories: preset and remodeling. Preset genes are those in which the binding sites for trans‐acting factors are accessible (;i.e. in a non‐nucleosomal, DNase I hypersensitive configuration) prior to activation. In response to the activation signal, positive factors bind to cis‐acting regulatory elements and trigger transcription with no (...) major alterations in the chromatin structure of the promoter region. In contrast, remodeling genes are those in which some of the required cis‐acting regulatory elements are packaged into nucleosomes. The nucleosomes must be perturbed in response to an activation signal in order for the trans‐acting factors to gain access to cis‐acting elements; a chromatin remodeling process which forms DNase I hypersensitive sites must occur. In both cases, precise positioning of nucleosomes along the promoter region of a gene appears to be critical for appropriate regulation of expression. (shrink)

Despite the developing influence of the Internet as a tool for reaching potential subjects, little empirical information exits on how individuals are recruited to participate in clinical research via the Internet or on what type of information clinical trial Web sites provide. This study revealed that roughly half of the sites failed to mention study risks or specific details about what the study required on the part of participants, while nearly three-quarters described incentives to participate. Moreover, for-profit entities (...) were more likely to be the sponsors of Web sites that did not appear to provide balanced information. These data suggest that policy-makers and IRBs should increase oversight of current online recruitment practices. (shrink)

A feature of the agro-industrial sphere is the high probability of distribution of production or research sites in areas far from each other for a considerable distance. Moreover, the center for collecting information and processing it, as a rule, is concentrated in one compact place. For research institutions, this feature often acquires a state of rather urgent problem, which requires the search for new innovative approaches. The paper proposes elements of the concept of construction and technological solutions for methods (...) of operational data transfer of field research from agricultural areas to a remote database for data storage with the possibility of feedback. As an example, the procedure of qualification examination of plant varieties with the definition of the criteria of "difference, homogeneity and stability" and "suitability for propagation of the variety in Ukraine" was chosen. (shrink)

A feature of the agro-industrial sphere is the high probability of distribution of production or research sites in areas far from each other for a considerable distance. Moreover, the center for collecting information and processing it, as a rule, is concentrated in one compact place. For research institutions, this feature often acquires a state of rather urgent problem, which requires the search for new innovative approaches. The paper proposes elements of the concept of construction and technological solutions for methods (...) of operational data transfer of field research from agricultural areas to a remote database for data storage with the possibility of feedback. As an example, the procedure of qualification examination of plant varieties with the definition of the criteria of "difference, homogeneity and stability" and "suitability for propagation of the variety in Ukraine" was chosen. (shrink)

This thesis was a critique of Hegel from a Heideggerian standpoint focusing on the role of action in community. It argues, first, that Heidegger has a more highly developed account of the present of action than does Hegel on account of his theory of temporality. On the basis of a discussion of the nature of action and its site, I examine the way in which action functions in community in both Hegel and Heidegger. For Hegel, action is essential to community; (...) intersubjective relations and mutual recognition depend upon it. For Heidegger, it is precisely the non-acting moment, the moment of vision (Augenblick), which forms the basis for community. Community, for Heidegger, would thus be prior to action, issuing from a place proper to Dasein which precedes all action in the world. I argue that the existence of a moment, the moment of vision, which would initially seem to refuse community in Heidegger actually provides a rich sense of the intersubjective because it allows for the experience of that which is totally Other. On the basis of this experience, Dasein -- the being that each and every one of us is -- would thus be capable of recognizing the essential alienness of the everyday communal world to it. Dasein would thus be able to recognize its difference and thus the difference of others from how their actions are taken up in the communal sphere. Action would thus not be the basis for community. For Hegel, action is essential to mutual recognition, which is the fabric of community. The radical otherness that is found in Heidegger when he articulates the authentic moment would be dismissed by Hegel as an affront to the possibility of community (Hegel’s beautiful soul). For Hegel, we are what we do and we are recognized exclusively on the basis of our actions. Consequently, I argue that Heidegger has a deeper and more radical sense of community than Hegel. This dissertation was inspired by the work of Maurice Blanchot. (shrink)

This thesis is a critique of Hegel from a Heideggerian standpoint focusing on the role of action in community. It argues, first, that Heidegger has a more highly developed account of the present of action than does Hegel on account of his theory of temporality. On the basis of a discussion of the nature of action and it's site, I examine the way in which action functions in community in both Hegel and Heidegger. For Hegel, action is essential to community (...) and intersubjective relations and mutual recognition depends upon it. For Heidegger it is precisely the non-acting moment, the moment of vision, which forms the basis for community. Community, for Heidegger would thus be prior to action, issuing from a place proper to Dasein which precedes all action in the world. I argue that the existence of a moment, the moment of vision, which would initially seem to refuse community in Heidegger actually provides a rich sense of the intersubjective because it allows for the experience of that which is totally Other. On the basis of this experience, Dasein would thus be capable of recognizing the essential alienness of the everyday communal world to it. It would thus be able to recognize its difference and thus the difference of others from how their actions are taken up in the communal sphere. Action would thus not be the basis for community. For Hegel, action is essential to the mutual recognition which is the fabric of community. The radical otherness that is found in Heidegger when he articulates the authentic moment is dismissed as an affront to the possibility of community. For Hegel, we are what we do and we are recognized exclusively on the basis of our actions. Consequently, I argue that Heidegger has a deeper and more radical sense of community than Hegel. (shrink)

Although 80–90% of adults are seropositive for antibodies against the human parvovirus adeno‐associated virus (AAV), infection has not been associated with either symptoms or disease. In cell culture, AAV infection is not productive unless there is a coinfection with a helper virus, either adenovirus or any type of herpes virus; in the absence of a helper virus coinfection the viral genome is integrated into the genome, usually at a specific site on chromosome 19q13.3‐qter. The integrated genome can be activated and (...) rescued by subsequent super infection by a helper virus. The high frequency of site‐specific integration by AAV and the lack of associated disease have encouraged the use of AAV as a vector for gene therapy. This review will focus on the molecular mechanisms involved in the establishment of, and rescue from, the latent state and their relevance to use of AAV as a vector. (shrink)

Investigation of metal is important for understanding relationship between production and ideology in ancient Near East. Metal production in the Eneolithic and Early Bronze Age stimulated transformation of egalitarian society into stratified one. The author traces relation of objects of social and religious significance (interior decorations, anthropo- and zoomorphic sculpture, symbolic weapons and implements) with certain types of sites. From the Neolithic onward metal was used in mortuary practice. In the Early Bronze Age metal production shows relationship with the (...) process of urbanization and formation of early polities. The Middle Bronze Age sees dramatic rise of metal production; valuable and symbolically important finds originate from royal tombs, temples, and treasures. When considering metal finds’ context, the author concludes that in the Near East in the late prehistory, urban civilization, and early states metal actively functioned in sacral sphere. Moreover, ideology to a great extent determined development of metal production. (shrink)

Although bone development during embryogenesis and bone repair after injury have a number of features which appear similar, they are distinctly different processes which involve separate controlling elements and cuing parameters. Repair of bone is influenced by bioactive factors which reside in bone itself; some of these factors are not present when embryonic mesenchymal cells first differentiate. For example, a bone protein which induces the conversion of mesenchymal cells into cartilage cells is not present in the embryo at the site (...) where cartilage first differentiates into the anlagen for future bone. Ultimately, the sequence of cellular and molecular events which controls the success of bone repair is dependent on the prior embryologic and developmental packaging of bioactive factors into bone and the release of these factors at a bone break site. The success of the reparative events depends not only upon the release of these bone‐derived factors but also on systemic factors and the identity and numbers of responding cells at the repair site. (shrink)

This article engages with the religious dimension of the politics of anti-slavery/trafficking and presents an analysis of select Christian-identified organisations working in anti-slavery/trafficking in South Africa. Using website content of the select organisations as primary material, the article argues that in similar ways to the paternalistic early Christian missionary approach to indigenous religious practices, the politics of paternalism persist to this day in the realm of Christian organisations working in anti-slavery in South Africa. That is, the ‘White Saviour Industrial Complex’ (...) identified by Teju Cole is pervasive in the rhetoric of these Christian organisations. Consequently, and firstly, the article further bolsters the argument that the African continent has frequently been treated as a key site for formulating and testing a variety of models of humanitarian assistance, aid and economic development dating from the colonial era to the present neocolonial one. However, and secondly, the article also highlights that there is ambivalence in the ways in which the Christian anti-slavery/trafficking organisations in South Africa take up a key feminist concept of empowerment as informing how they understand their work. As such, the article proposes that caution be applied in critically engaging the discourse of these organisations.Contribution: This article engages with the religious dimension of the politics of anti-slavery/trafficking and presents an analysis of select Christian-identified organisations working in anti-slavery/trafficking in South Africa. The article illustrates how the intersection of political studies and religion is part of the journal’s focus on interdisciplinary, multidisciplinary and transdisciplinary contested discourse. (shrink)

The Athenian expedition led against Macedonia by Archestratos, son of Lykomedes, early in 432 was not diverted from its destination by the revolt of Poteidaia. Archestratos had received additional instructions to enforce the Poteidaia ultimatum if he could, but, this being already impossible, he continued with the real object of his mission, the attack on Perdikkas II of Macedon. The widespread revolt among the Chalkidians had deprived the Athenians of the bases for this attack on which they might have reckoned, (...) and Archestratos had at the outset to make good this loss by recapturing Therme, at the head of the gulf to which it gave its name. Therme, or Serme, had been a tributary member of the Delian League since 450/449:2 it need not necessarily be said that it lay within Macedonia, as Poppo and Bergk inferred from Thucydides 1. 61. 2, but it is at least likely that it lay on the boundary, as it was handed over to Perdikkas under the agreement of 431 .3 However, that the Athenians could include in their Empire a city within Macedonian territory is shown from the position of Berge , and is likely in other cases, e.g. Haison. From Therme, Archestratos moved on to Pydna, presumably by sea, and laid siege to it. Here he was joined by substantial reinforcements, while at the same time the situation in Chalkidike became increasingly embarrassing, so that a peace and a reinsurance alliance were seen by both sides to be ‘imperative’ . When these had been concluded, the Athenians moved against Poteidaia. (shrink)

The extracellular matrix does more than just blanket cells; it also provides informational cues which affect a variety of developmental and cellular maintenance activities. The constituents of the matrix provide the fabric for cell motility and cell shape as well as anchorage sites for bioactive factors which directly affect the cell's developmental pattern or mitotic activity. The influence of the extracellular matrix is controlled by the cell's responsiveness to these complex signals. The same matrix component, for example hyaluronic acid, (...) can have completely different effects depending on the cell's lineage and developmental history. The functional interaction between the extracellular matrix and specific cell surface receptors provides cues which affect the control of development and the maintenance and aging events that affect specific cells and tissues. (shrink)

We have synthesized a 582,970-base pair Mycoplasma genitalium genome. This synthetic genome, named M. genitalium JCVI-1.0, contains all the genes of wild-type M. genitalium G37 except MG408, which was disrupted by an antibiotic marker to block pathogenicity and to allow for selection. To identify the genome as synthetic, we inserted "watermarks" at intergenic sites known to tolerate transposon insertions. Overlapping "cassettes" of 5 to 7 kilobases (kb), assembled from chemically synthesized oligonucleotides, were joined by in vitro recombination to produce (...) intermediate assemblies of approximately 24 kb, 72 kb ("1/8 genome"), and 144 kb ("1/4 genome"), which were all cloned as bacterial artificial chromosomes in Escherichia coli. Most of these intermediate clones were sequenced, and clones of all four 1/4 genomes with the correct sequence were identified. The complete synthetic genome was assembled by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae, then isolated and sequenced. A clone with the correct sequence was identified. The methods described here will be generally useful for constructing large DNA molecules from chemically synthesized pieces and also from combinations of natural and synthetic DNA segments. 10.1126/science.1151721. (shrink)

In lieu of an abstract, here is a brief excerpt of the content:The University and Democracy: A Response to “Dewey, Implementation, and Creating a Democratic Civic University”Lee A. McBride IIIira harkavy has given us much to consider. His paper, “Dewey, Implementation, and Creating a Democratic Civic University,” invites us to critically assess our democracy and the role of colleges and universities in the propagation of our democratic way of life. Harkavy suggests that universities are failing to fulfill their function, that (...) is, “to educate students to be ethical, empathetic, engaged, democratic citizens, and advance knowledge for the continuous betterment of the human condition” (50). He suggests that universities are central societal institutions in the world, and research universities are most central—“they develop new ideas and technologies, incubate businesses, serve as cultural and artistic centers, and are engines of local, national, and global economics” (50). Our universities produce our teachers and our teachers’ teachers; they shape the learning, values, and the aspirations of students (Harkavy 50). But, again, universities are not fulfilling their social function of “creating and sustaining an inclusive, just democratic society” (Harkavy 51). Harkavy boldly asserts that a revolutionary change is required in higher education if it is to fulfill its democratic function.Closed LoopI have a concern about the role of Research 1 (R1) universities in this picture. I am led to believe that R1 universities, like the University of Pennsylvania, exist in rarefied air. Penn, for example, has a ridiculous endowment—more than $20 billion. Penn apparently accepts only 9% of its applicants. The resources and demographics of R1 institutions are unlike those at state schools and small liberal arts colleges. The various canons, the faculty, and the student body at elite R1 universities have historically represented the thoughts, [End Page 76] experiences, and perplexities of well-heeled white heteronormative men. The vast majority of the faculty and students at R1 universities are predominantly of Anglo-European descent and a higher socioeconomic status. This gives me pause. If R1 universities are placed as the central engines of democratic fomenting, I worry that the faculty and students at R1 universities will direct research and social amelioration projects in particular directions and retain particular presuppositions that will buttress the continued flourishing of elite R1 universities. A closed loop. I worry that both the material and the social capital needed to (re)shape social conditions will arise only from and circulate only between those who attend elite R1 universities. I mean, Harkavy suggests that the departments, programs, and institutes at R1 universities create and sustain those knowledges and skills that produce the scholars and thoughtful people who will drive our K–12 education, who will fashion new cooperatives and entrepreneurial ventures, and who will become our community activists and political leaders. As such, the knowledge and skills to implement a more just and inclusive democracy will be disseminated from the R1 university. Harkavy also explains that it is necessary that universities strive to be democratic civic universities, sites where university folk work alongside members of the surrounding communities to address and ameliorate the problems on the ground. This “would involve significant and ongoing engagement of an institution’s comprehensive assets (academic, human, cultural, and economic) in partnership with community members to produce knowledge and educate ethical students with the ability to help create and maintain just, anti-racist, democratic societies” (Harkavy 53). The democratic civic university would then nurture a culture of democracy that exceeds mere voting practices— democracy as a way of life (Dewey 226). Prima facie, this seems like a noble goal. And yet, I still worry that those university folks who do this work will be a small representative group of people who bear particular life experiences and perhaps phenotypic markers.I would suggest that other sites outside of the R1 university (e.g., the trade union meeting, the settlement house, the church, the community garden, etc.) will need to be taken as legitimate sites of knowledge creation or legitimate sites of communication and joint activity (Collins 269). This would at least bring differing life experiences to the table; that is, it would bring differing life experiences to the fore and recognize alternative non-academic sources of insight and wisdom. These sites and alternative... (shrink)

As researchers, we cannot be outside society and thus activities such as "science," or "objectivity" are striated with procedures for minimizing or celebrating the presence of the researcher in the research product. Our recognition of the situated character of scientific knowledge is the context in which questions about the researchers relation to the group she studies have arisen. The paper begins with a review of the Insider/Outsider debate which circles around the researcher''s relation to those she studies. Where the researcher (...) enters the research site as an Insider - someone whose biography (gender, race, class, sexual orientation and so on) gives her a lived familiarity with the group being researched - that tacit knowledge informs her research producing a different knowledge than that available to the Outsider - a researcher who does not have an intimate knowledge of the group being researched prior to their entry into the group. This paper describes the research issues that arise and the various strategies researchers have used to manage them. The argument then shifts to query the social boundaries implicit in the construction of research Insiders and Outsiders. Reflecting on research that explored mothering for schooling, the article shows that researchers are rarely Insiders or Outsiders. Rather, research is constructed in a relationship with many Others. The interaction of individual biography and social location shape the research relation in complex ways which undercut the common-sense translation of historical familiarity into epistemological privilege. (shrink)

This study examines the tension between the academic evaluation system and the development of unconventional research agendas. While scholars have studied the evaluation of research that crosses disciplinary boundaries from the perspective of peer reviewers, they have paid comparatively little attention to the experiences of the performers of unconventional science. This study asks how researchers develop unconventional research agendas to address a long-standing health problem and, in the process, make sense of the actions of a site visit committee organized to (...) advise a foundation funding the project. This study develops a process narrative on the development of a specific transdisciplinary team proposing to study premature birth. The findings show that when the performers and the evaluators of unconventional science developed competing understandings of the research agenda, transdisciplinary discovery became limited as a particular research topic became taboo. Yet, the study also reveals how the performers of unconventional science challenged the power of the site visit committee by making it seem as though they followed the committee’s decisions. These findings raise questions about the role of private foundations as funders of academic research and the suitability of traditional evaluation procedures for assessing transdisciplinary discovery. (shrink)

Abstract:This essay examines the experiences of three 19th-century African American travelers to Greece—David Dorr (1852), Frederick Douglass (1887), and John Wesley Gilbert (1890–1)—using evidence from their letters, diaries, and published writings. The essay shows that although each traveler's unique personal perspective shaped his response to seeing the ancient sites and monuments of Greece, all three men responded most deeply to a site connected with Greece's Christian heritage: the Areopagus or Mars Hill, where according to 19th-century understanding the Apostle Paul (...) had spoken the words recorded in the Book of Acts. (shrink)

Initial responses to questionnaires used to assess participants' understanding of informed consent for malaria vaccine trials conducted in the United States and Mali were tallied. Total scores were analyzed by age, sex, literacy (if known), and location. Ninety-two percent (92%) of answers by United States participants and 85% of answers by Malian participants were correct. Questions more likely to be answered incorrectly in Mali related to risk, and to the type of vaccine. For adult participants, independent predictors of higher scores (...) were younger age and female sex in the United States, and male sex in Mali. Scores in the United States were higher than in Mali (P = 0.005). Despite this difference participants at both sites were well informed overall. Although interpretation must be qualified because questionnaires were not intended as research tools and were not standardized among sites, these results do not support concerns about systematic low understanding among research participants in developing versus developed countries. (shrink)

Larry Temkin famously argues that what he calls spectrum arguments yield strong reason to reject Transitivity, according to which the ‘all-things-considered better than’ relation is transitive. Spectrum arguments do reveal that the conjunctions of independently plausible claims are inconsistent with Transitivity. But I argue that there is very strong independent reason to reject such conjunctions of claims, and thus that the fact that they are inconsistent with Transitivity does not yield strong reason to reject Transitivity.

Automatic incident detection plays a vital role among all the safety-critical applications under the parasol of Intelligent Transportation Systems to provide timely information to passengers and other stakeholders in smart cities. Moreover, accurate classification of these incidents with respect to type and severity assists the Traffic Incident Management Systems and stakeholders in devising better plans for incident site management and avoiding secondary incidents. Most of the AID systems presented in the literature are incident type-specific, i.e., either they are designed for (...) the detection of accident or congestion. While traveling along the road, one may come across different types of traffic incidents, such as accidents, congestion, and reckless driving. This necessitates that the AID system detects and classifies not only all the popular traffic incident types, but severity as well that is associated with these incidents. Therefore, this study aims to propose an efficient incident detection and classification framework for smart cities, by incorporating the efficacy of model stacking, to classify the incidents with respect to their types and severity levels. The experimental results showed that the proposed E-IDC framework achieved performance gains of 5%–56% in terms of incident severity classification and 1%–14% in terms of incident type classification when applied with different classifiers. We have also applied the Wilcoxon test to benchmark the performance of our proposed framework that reflects the significance of our approach over existing individual incident predictors in terms of severity and type classification. Moreover, it has been observed that the proposed E-IDC framework outperforms the existing ensemble technique, such as XGBoost used for the classification of incidents. (shrink)

The idea of the “Active education” means the opportunity for children to study the surrounding world not only by textbooks or with the help of the sites in the INTERNET (a rather passive action) but also by a direct contact with this world via its active investigation and solution of some concrete problems.This may be the study of environmental contamination and other modern practical ecological problems such as the transformation of the agriculture production resulting from climatic variations or anthropogenic (...) changes of landscapes. (shrink)

As an international IRB collaboration project, we set out to develop an approach to enhance the understanding of issues related to the conduct of human research with an international partner. While the larger project included two specific aims, the activities supporting the first aim are addressed in this paper—specifically, human research protection program capacity strengthening in Cali, Colombia, through the development of an infrastructure that supports the conduct of human research with appropriate protection of subjects and access to new resources (...) at the international site. (shrink)

Summary Using the neurological syndrome kuru as a frame, Warwick Anderson examines the social dynamics and material culture of its medical investigation among the Fore people conducted by D. Carleton Gajdusek beginning at midcentury. The Collectors of Lost Souls: Turning Kuru Scientists into White Men uses a postcolonial framework to complicate dominant/subordinate binaries and diffusionist accounts of indigenous contacts with medical science. Anderson proposes that colonies are specific sites of production of medical knowledge. He draws a distinction between traditional (...) and advanced economies in regard to individual rights to make an argument in support of context-specific ethical regulation for medical research and suggests a role for historians of science in interrogating the process of globalisation. (shrink)

Ensuring that clinical trials, once launched, successfully complete and generate useful knowledge is an important and indeed ethically imperative goal, given the risks and burdens borne by research participants. Since there are insufficient willing research participants to power all the trials that are currently undertaken,1 addressing underenrolment will require prioritisation decisions that reduce the number of trials competing for participants. While there are multiple levels at which research priority-setting can and does take place, competition between trials often plays out in (...) real time at the institutional or site level, where complex decisions must be made about how to manage overlapping trials in ways that balance different considerations, including the risk of non-completion. We sought to explore what research institutions in particular might ethically do to mitigate the risk that competition between trials will contribute to recruitment shortfalls. Against this backdrop, we appreciate the thoughtful replies to our article and are especially encouraged that all three respondents acknowledge the importance and indeed necessity of setting research priorities in ways that respect the rights and interests of various parties. The key question raised by the commentaries primarily concerns not whether research prioritisation should take place but rather how it is best accomplished. In what follows, we clarify our argument in the original article, and then focus on several points raised in the commentaries regarding the role of institutions in research priority-setting. Our approach is animated by the risk that competition between clinical trials for the same population of participants can be a cause of underenrolment when there are insufficient participants to meet the statistical needs of all open studies. In such situations, one or more of the competing studies will fail to meet recruitment targets, reducing their statistical ability to answer the research question. There are strong ethical reasons to avoid …. (shrink)

Background: Managing HIV treatment is a complex multi-dimensional task because of a combination of factors such as stigma and discrimination of some populations who frequently get infected with HIV. In addition, patient-provider encounters have become increasingly multicultural, making effective communication and provision of ethically sound care a challenge. Purpose: This article explores ethical issues that health service providers in the United States and Botswana encountered in their interaction with patients in HIV care. Research design: A descriptive qualitative design was used (...) to collect data from health service providers and patients using focused group discussions. This article is based on responses from health service providers only. Participants and context: This article is based on 11 focused group discussions with a total sample of 71 service providers in seven US sites and one Botswana site. Ethical considerations: Ethical review boards at all the study sites reviewed the study protocol and approved it. Ethical review boards of the study’s coordinating centers, Rutgers University and the University of California at San Francisco, also approved it. The study participants provided a written informed consent to participate. Findings: HIV service providers encountered ethical challenges in all the four Beauchamp and Childress’ biomedical ethics of respect for patients’ autonomy, beneficence, justice, and nonmaleficence. Discussion: The finding that HIV service providers encounter ethical challenges in their interaction with patients is supported by prior studies. The ethical challenges are particularly prominent in multicultural care and resource-constrained care environments. Conclusion: Provision of HIV care is fraught with ethical challenges that tend to pose different issues depending on a given care environment. It is important that strong partnerships are developed among key stakeholders in HIV care. In addition, health service providers need to be provided with resources so they can provide quality and ethically sound care. (shrink)

Extreme Contemporary is a concise intellectual biography of Maurice Blanchot, a figure whose name, Leslie Hill claims, marks the site where the most important ideas of 19th and 20th century European philosophy overlap, intersect, and indeed, come to their fruition. It situates Blanchot as the radical heir to the questions concerning totality, experience, limit, Being, and Other, which G.W.F. Hegel and Martin Heidegger left in their wake, and it distinguishes him from George Bataille and Emmanuel Levinas, his friends and close (...) counterparts. It does justice to the subtlety and elusive quality of Blanchot's writing. (shrink)

Cahokia, the largest pre-European settlement in North America, was situated on the Middle Mississippi River floodplain and flourished for approximately three hundred years from the 10th century AD onward. The site was favorably located from an environmental standpoint, being proximal to a diversity of microhabitats including expanses of open water and marshes from which the essential, renewable fish protein could be procured. More importantly, the largest local zone of soils characterized as optimal for prehistoric hoe cultivation lay immediately to the (...) east. Here, on the floodplain and along its bordering alluvial fans, the large maize outfields were situated, while the multi-crop house gardens were placed within the habitation zone on soils that had often been culturally enriched by prior occupation. As successful as this strategy might have been for small, dispersed populations in such a plentiful environment, nucleation of large numbers of people at Cahokia provided a different adaptive context that ultimately led to ruinous consequences. The seeds for the city's destruction centered anthropogenically produced environmental degradation. Demands on wood resources for fuel and construction were enormous and agricultural field clearance was in forested rather than prairie settings. The resultant watershed deforestation produced greatly increased rates of erosion, runoff, and unseasonable downstream flooding during the summer growing season. The economic and social consequences of declining production and localized crop failures proved disastrous for this city of farmers. (shrink)

This article offers a series of critical theorizations on the biopolitical dimensions of methicillin-resistant Staphylococcus aureus (MRSA), with specific attention to what has recently been referred to in the United States as the ‘MRSA Epidemic’. In particular, we reflect on the proliferation of biomedical discourses around the ‘spread’, and the pathogenic potentialities, of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA). We turn to the work of Roberto Esposito and Jean-Luc Nancy to better make sense of how, during this immunological crisis, the individualized (...) fleshy and fluid body is articulated to dimensions of community and corporeal proximity; the body is thus conceived in popular biopolitical framings as a site of transmission, inoculation, and isolation – as a living ecological and pathological vessel. We give emphasis to the spatial relations of flesh, namely in how biomedical ‘experts’ have sought to (bio-)technologize spaces of heightened communal bodily contact (such as playgrounds or gymnasia). (shrink)

The actin cytoskeleton is a key cellular structure subverted by pathogens to infect and survive in or on host cells. Several pathogenic strains of Escherichia coli, such as enteropathogenic E. coli (EPEC) and enterohemorrhagic E. coli (EHEC), developed a unique mechanism to remodel the actin cytoskeleton that involves the assembly of actin filament‐rich pedestals beneath the bacterial attachment sites. Actin pedestal assembly is driven by bacterial effectors injected into the host cells, and this structure is important for EPEC and (...) EHEC colonization. While the interplay between bacterial effectors and the actin polymerization machinery of host cells is well‐understood, how other mechanisms of actin filament remodelling regulate pedestal assembly and bacterial attachment are poorly investigated. This review discusses the gaps in our understanding of the complexity of the actin cytoskeletal remodelling during EPEC and EHEC infection. We describe possible roles of actin depolymerizing, crosslinking and motor proteins in pedestal dynamics, and bacterial interactions with the host cells. We also discuss the biological significance of pedestal assembly for bacterial infection. (shrink)

The specificity of eukaryotic DNA organization into loops fixed to the nuclear matrix/chromosomal scaffold has been studied for more than fifteen years. The results and conclusions of different authors remain, however, controversial. Recently, we have elaborated a new approach to the study of chromosomal DNA loops. Instead of characterizing loop basements (nuclear matrix DNA), we have concentrated our efforts on the characterization of individual loops after their excision by DNA topoisomerase II‐mediated DNA cleavage at matrix attachment sites. In this (...) review the results of applying this mapping approach are compared with the results and conclusions from studies of nuclear matrix DNA. An attempt is also made to reconsider all data about the specificity of DNA interactions with the nuclear matrix and to suggest a model of spatial organization of the eukaryotic genome which resolves apparent contradictions between these data. (shrink)

This essay presents a critical appraisal of the current state of baccalaureate Health Humanities, with a special focus on the contextual differences currently influencing the implementation of this field in Canada and, to a lesser extent, the United States and United Kingdom. I argue that the epistemological bedrock of Health Humanities goes beyond that generated by its written texts to include three external factors that are especially pertinent to undergraduate education: site (the setting of Health Humanities education), sector (the disciplinary (...) eligibility for funding) and scope (the critical engagement with a program’s local context alongside an emergent “core” of Health Humanities knowledge, learning, and practice). Drawing largely from the Canadian context, I discuss how these differences can inform or obstruct this field’s development, and offer preliminary recommendations for encouraging the growth of baccalaureate Health Humanities—in Canada and elsewhere—in light of these factors. (shrink)

We have now had some two decades of Levinas commentary. What remains to be said? Certainly one thing we have learned since Otherwise than Being is that Levinas’s philosophy and his talmudic and confessional writings nourish each other so profoundly that to approach Levinas without understanding the historyof Jewish philosophy — in its confrontations with neo-Platonism, Aristotle, Kant — is to risk misunderstanding Levinas. Insights into the interrelationships between Jewish thought and Levinas’s other humanism have been provided by thinkers like (...) Robert Gibbs, Claire Katz, Catherine Chalier, Shmuel Trigano, and Gérard Bensussan, to name but a few. But if one is not well versed in Jewish thought, will one be liable to abandon Levinas’s thought as an existentialized confessionalism? Perhaps. But I think the loss to philosophy would be considerable. (shrink)

Cyclin-dependent kinase 5 has been implicated in Alzheimer's disease pathogenesis. Here, we demonstrate that overexpression of p25, an activator of cdk5, led to increased levels of BACE1 mRNA and protein in vitro and in vivo. A p25/cdk5 responsive region containing multiple sites for signal transducer and activator of transcription was identified in the BACE1 promoter. STAT3 interacts with the BACE1 promoter, and p25-overexpressing mice had elevated levels of pSTAT3 and BACE1, whereas cdk5-deficient mice had reduced levels. Furthermore, mice with (...) a targeted mutation in the STAT3 cdk5 responsive site had lower levels of BACE1. Increased BACE levels in p25 overexpressing mice correlated with enhanced amyloidogenic processing that could be reversed by a cdk5 inhibitor. These data demonstrate a pathway by which p25/cdk5 increases the amyloidogenic processing of APP through STAT3-mediated transcriptional control of BACE1 that could have implications for AD pathogenesis. (shrink)

The aim of this study was to carry out a psychosocial analysis of child-to-parent violence (CPV) in a sample of school adolescents, considering a set of individual variables (psychological distress, problematic use of social networking sites, and perceived non-conformist social reputation) and family variables (open and problematic communication with parents) according to sex. The sample consisted of 3,731 adolescents (54% boys), aged between 14 and 16 years (M = 14.6 years, SD = 0.567), from the state of Nuevo León, (...) Mexico. The scores of the boys and girls were analyzed to check for differences. Also, correlations between all the study variables were calculated. Finally, a multiple stepwise regression analysis was carried out for the total sample and also for boys and girls separately. Results confirmed the important role of individual variables as predictors of CPV in boys and girls. The main difference between boys and girls was observed in the predictive weight of problematic use of social networking sites, which was higher in girls than in boys. Open communication with the father was a significant factor for predicting the decrease of CPV levels in the case of boys, while open communication with the mother predicted the decrease of CPV in girls. Problematic communication with the mother showed similar values in boys and girls when predicting CPV, however, the predictive weight of problematic communication with the father was higher in girls than in boys. These results are interesting and have important implications for the prevention of CPV. (shrink)

The mitochondrion is known as the “powerhouse” of eukaryotic cells since it is the main site of adenosine 5′‐triphosphate (ATP) production. Using a temperature‐sensitive fluorescent probe, it has recently been suggested that the stray free energy, not captured into ATP, is potentially sufficient to sustain mitochondrial temperatures higher than the cellular environment, possibly reaching up to 50 °C. By 50 °C, some DNA and mitochondrial proteins may reach their melting temperatures; how then do these biomolecules maintain their structure and function? (...) Further, the production of reactive oxygen species (ROS) accelerates with temperature, implying higher oxidative stresses in the mitochondrion than generally appreciated. Herein, it is proposed that mitochondrial heat shock proteins (particularly Hsp70), in addition to their roles in protein transport and folding, protect mitochondrial proteins and DNA from thermal and ROS damage. Other thermoprotectant mechanisms are also discussed. (shrink)

In real life, listening to music may be associated with an eyes-closed or eyes-open state. The effect of eye state on listeners’ reaction to music has attracted some attention, but its influence on brain activity has not been fully investigated. The present study aimed to evaluate the electroencephalographic (EEG) markers for the emotional valence of music in different eye states. Thirty participants listened to musical excerpts with different emotional content in the eyes-closed and eyes-open states. The results showed that participants (...) rated the music as more pleasant or with more positive valence under an eyes-open state. In addition, we found that the alpha asymmetry indices calculated on the parietal and temporal sites reflected emotion valence in the eyes-closed and eyes-open states, respectively. The theta power in the frontal area significantly increased while listening to emotional-positive music compared to emotional-negative music under the eyes-closed condition. These effects of eye states on EEG markers are discussed in terms of brain mechanisms underlying attention and emotion. (shrink)

Many individuals on the autism spectrum are hypersensitive to certain sensory stimuli. For this group, as well as for non-autistic individuals with sensory processing disorders, being exposed to e.g. fluorescent lights, perfume odours, and various sounds and noises can be real torment. In this article, I consider the normative implications of such offence for the design of office spaces, which is a topic that has not received any attention from philosophers. After identifying different ways in which the senses of (...) hypersensitive workers might be protected within these spaces, I show that many of such accommodations can be made at reasonable cost, before arguing that doing so ought to be a legal requirement. (shrink)

Social opposition to instituted policies and practices marks the sites of resistance that populate the contemporary political landscape. Animated by the prospects of a better and more just world, the emancipatory ambitions of social and political movements bring to the fore discrepancies between ideologically congealed power relations and habits of thought and the subversive function of utopian expectations. Paul Ricoeur reminds us that our participation in society is invariably punctuated by our experiences of reality’s noncongruence with imaginative alternatives we (...) can project and upon which we can act. After explaining how literary fictions open spaces for reworking reality, I set out the imagination’s analogous power on the political plane. The struggles with which social and political movements are engaged seek to transform established conventions. Hence, like literary works, these movements aim at refashioning the existing order of reality from within. Protest movements attest to how struggles for recognition combat systemic injustices by holding out the prospect of a different and better future. Consequently, these movements exemplify the power that springs from individuals acting in consort, as evidenced by recent protests against the Trump administration. Conversely, violence destroys power. In view of the way that future expectations animate the force of the present, I therefore argue that dystopic representations of authoritarian regimes in Margret Atwood’s The Handmaid’s Tale and George Orwell’s 1984 fulfill a critical, social function as apocalyptic harbingers of political corruption and deceit. As such, these dystopian novels intensify the force that the present has as a time of crisis and decision. (shrink)

Ulwaluko is an essential rite of passage for men in the Xhosa community in South Africa. This particular initiation rite of passage is a critical part of the transition from boyhood to manhood. In a post-apartheid South Africa, this African practice has received criticism for promoting gender and sexual exclusion with some calling for a halt to the practice. Using Simon Maimela’s theological anthropology and Jackson’s concept of modern slavery as human plasticity, I attempt to expose how post-apartheid constitutionalism has (...) epistemically distorted the theological anthropology of this rite of passage. This is done acknowledging Ulwaluko theologically within the context of African holism. This, I argue, has had significant ethical implications for society. Recognising it as a site of black theological reflection using Maimela’s theological anthropology, I argue that this particular practice can positively influence discourses on morality in South Africa.Contribution: This article makes a theological contribution by situating African and black theology of liberation discourse within the epistemic framework of Ulwaluko as an initiation rite of passage. Recognising the prevalence of initiation rites of passage in South Africa, this discourse attempts to contribute towards thinking about the creation of humanity from these spaces of formation. This I do in the very challenging contexts within which Ulwaluko is practised. (shrink)

Understanding the unique status of uncommemorated trauma sites requires questioning the practice of referring to such sites solely as "mass graves." Indeed, it is the fact that the people once thrown into the pits have never been buried that generates today's ambivalent memory of the past associated with a given place. The unburied—in grassroots perception—threaten social homeostasis. I compare the findings of anthropologists regarding burial practices with the knowledge provided today by forensic/conflict archaeologists and ethnographers, indicating the special (...) status of sites containing unritualized bodies of victims. This more nuanced approach, differentiating "mass graves" from "burial pits" will allow for more complex readings of contemporary interactions with a site, exhibited by local "implicated" bystander communities. Eastern European Holocaust landscapes especially, may thus be open to renewed scrutiny. (shrink)