We have synthesized a 582,970-base pair Mycoplasma genitalium genome. This synthetic genome, named M. genitalium JCVI-1.0, contains all the genes of wild-type M. genitalium G37 except MG408, which was disrupted by an antibiotic marker to block pathogenicity and to allow for selection. To identify the genome as synthetic, we inserted "watermarks" at intergenic sites known to tolerate transposon insertions. Overlapping "cassettes" of 5 to 7 kilobases (kb), assembled from chemically synthesized oligonucleotides, were joined by in vitro recombination to produce intermediate (...) assemblies of approximately 24 kb, 72 kb ("1/8 genome"), and 144 kb ("1/4 genome"), which were all cloned as bacterial artificial chromosomes in Escherichia coli. Most of these intermediate clones were sequenced, and clones of all four 1/4 genomes with the correct sequence were identified. The complete synthetic genome was assembled by transformation-associated recombination cloning in the yeast Saccharomyces cerevisiae, then isolated and sequenced. A clone with the correct sequence was identified. The methods described here will be generally useful for constructing large DNA molecules from chemically synthesized pieces and also from combinations of natural and synthetic DNA segments. 10.1126/science.1151721. (shrink)

This volume explores worldwide developments in the field of business ethics. It studies ethical issues faced by transnational corporations, the possibilities for international cooperation after the cold war, as well as regional business ethics issues from around the world. The essays, taken from the Ninth Bentley Conference on Business Ethics sponsored by the Center for Business Ethics, include cases and regional studies from Africa, Eastern Europe, the Pacific Rim, and North and South America. Topics discussed include the BCCI scandal, the (...) IBM-Fujitsu case, land-use policies, transnational codes of business conduct, ethics and international law, business and cultural diversity, and ethics and economic development. (shrink)

In lieu of an abstract, here is a brief excerpt of the content:274 HISTORY OF PHILOSOPHY poisoning, spying, etc., which would render postwar mutual confidence impossible, shall not be countenanced. It is mainly with an eye to these preliminary articles that Professor Wilhelm Miiller argues for Kant's relevance to contemporary political problems. Miiller begins by drawing an analogy between the Peace of Basle (1795) and the Treaty of Versailles: in both instances, it is claimed, secret reservations at the treaty table, (...) the identification of defeated nation with "guilty" nation, and the deprivation of an entire "guiltless" people of "the most primitive right to live" helped lay the foundations for future wars. Miiller sees in the willful dispersion, through motives of revenge and greed, of the possessions of conquered nations after both World Wars a "reversion to the wildest barbarism." He defends Kant's stricture against standing armies as being "simply and logically irrefutable" in spite of Kant's toleration--understandable in 1795--of limited and voluntary militias adequate for national defense. He infers from Kant's warning against national economic dependency the urgent need to exclude international capitalism from world politics. He suggests that Kant's principle of non-interference in the internal affairs of nations has been violated in the splitting of East from West Germany. And he insinuates that the present form of government in the U.S. is democratic and republican only in name, being in actuality a capitalist oligarchy whose two-party system tends to degenerate into a one-party system. Professor Miiller is entitled to his views. The reader, however, is entitled to a more objective exposition of Kant's essay and perhaps even to hope for a more critical and searching appraisal of Kant's doctrines. One opportunity for the latter surely offers itself in regard to Kant's analogy between personal and international morality. The analogy might conceivably break down over the difficulty of assigning collective moral and legal responsibility. If, as Kant maintains, a state is a moral person, it should follow that it and not merely its constituent members taken singly has moral responsibility for what it undertakes. Leaving aside the question of whether or not Kant thought through the implications of his premise, it is clear that Professor Miiller has not done so. Whereas he considers atrocious the branding of the German people as guilty of international crime in either World War, he allows himself the judgment that the "Christian" people of Europe and America were guilty of mass-murder on both occasions. The inconsistency is evident. Left unexamined and even unmentioned are the following: Kant's well-known ethical principles--the concept of practical reason, the phenomenal-noumenal duality, the notion of design in nature, the doctrine of the Categorical Imperative binding on any rational will--upon which he explicitly grounds Perpetual Peace; his efforts to refute the notion of theoretical conflict between morality and politics; his misleading use of the language of the social contract theorists throughout the essay; his proposal, anticipatory of later British philosophers, that the principle of publicity is a necessary if not sufficient condition of morally proper legislation. Perpetual Peace deserves more than Professor Mfiller has given it. PETER FUSS University of California, Riverside The Earlier Letters of John Stuart Mill 1812-1848. Edited by Francis E. Mineka, with an Introduction by F. A. Hayek. Two vols. (Toronto: University of Toronto Press, Routledge g: Kegan Paul. Pp. xxvii + 784. $20.00.) These two volumes of early correspondence are the first volumes to appear of a projected new edition of Mill's collected works. If the subsequent volumes maintain the high level of competence achieved here, the new edition will prove a valuable assistance to nineteenth- BOOK REVIEWS 275 century scholars. Of the 537 letters published, 238 have never before been in print, and 72 others have hitherto appeared only in part. Most of the work of assembling the letters was done by F. A. Hayek, but the extremely useful annotation was prepared by Francis E. Mineka. We are told that he and Dwight N. Lindley are planning an edition of the later letters (1849-1873). The earliest letter extant (printed in facsimile and hitherto unpublished) was written, at the age of 6, to Bentham. It... (shrink)

When Seneca comes to describe the appearance of the monstrous bull which appears out of the sea to kill Hippolytus in answer to his father's curse, he uses a metaphor of birth: the sea's wave is said to be ‘heavy with burdened womb’ . If line 1016 is genuine – it was athetized by Leo – the sea is said to be ‘pregnant with a monster’ . The metaphor has not passed unnoticed in modern commentaries but it has not been (...) fully appreciated. I want to examine further linguistic aspects of the metaphor, and to consider its significance in its literary context. Seneca is a writer who likes – as he himself acknowledges – to re-work tradition: all the more significant, then, when he introduces a conspicuous metaphor into his text which does not appear to have literary antecedents. (shrink)

An elementary-particle picture developed primarily by Barut as an alternative to the standard model is re-examined. This model is formulated on the basis of strong short-range magnetic interactions among the stable particles (p, e−, v) and at present is able to account qualitatively for most of the known phenomena.

W. Mays; II—Carnap on Logic and Language, Proceedings of the Aristotelian Society, Volume 62, Issue 1, 1 June 1962, Pages 21–38, https://doi.org/10.1093/aristot.

W. H. F. Barnes; XI*—Referring to Individuals, Proceedings of the Aristotelian Society, Volume 73, Issue 1, 1 June 1973, Pages 167–180, https://doi.org/10.1093/.