“Every man his specialty” brings recent debates about dependency into the foreground of disability studies by looking at one modernist author, Samuel Beckett, whose characters are often disabled but who rely on each other for solace and support. Beckett’s plays explore the “abject dependence” of individuals for whom ontological and theological props have been removed and who must negotiate the passing of time in order as Estragon in Godot says, “to create the illusion we exist.

This volume studies a range of modernist works by writers such as Virginia Woolf, Oscar Wilde, Henry James, and James Joyce to explore what Modernism looks like when viewed through the lens of disability.

The discovery and engineering of novel fluorescent proteins (FPs) from diverse organisms is yielding fluorophores with exceptional characteristics for live‐cell imaging. In particular, the development of FPs for fluorescence (or Förster) resonance energy transfer (FRET) microscopy is providing important tools for monitoring dynamic protein interactions inside living cells. The increased interest in FRET microscopy has driven the development of many different methods to measure FRET. However, the interpretation of FRET measurements is complicated by several factors including the high fluorescence background, (...) the potential for photoconversion artifacts and the relatively low dynamic range afforded by this technique. Here, we describe the advantages and disadvantages of four methods commonly used in FRET microscopy. We then discuss the selection of FPs for the different FRET methods, identifying the most useful FP candidates for FRET microscopy. The recent success in expanding the FP color palette offers the opportunity to explore new FRET pairs. (shrink)