This paper examines the contribution of the PhD dissertation of the American cytologist Michael F. Guyer (1874-1959) to the early establishment (in 1902-1903) of the parallel relationship between cytological chromosome behaviour in meiosis and Mendel's laws. Guyer's suggestions were among the first, which attempted to relate the variation observed in the offspring in hybridisation studies by a coherent cytological chromosome mechanism to meiosis before the rediscovery of Mendel's principles. This suggested for the first time that the chromosome mechanism involved a (...) conjugation of maternal and paternal chromosomes during the synapsis followed by a segregation of parental chromosomes in the final germ cells and a random union of the final germ cells in the fertilization. It shows that this early suggestion was similar to William Austin Cannon's later chromosome proposal attempting to explain Mendel's principles and had some influence on Walter Sutton's cytological suggestion explaining correctly the behaviour of Mendel's particle by 1903. (shrink)

Neuronal aggregates involved in conscious awareness are not evenly distributed throughout the CNS but comprise key components referred to as the neural network correlates of consciousness (NNCC). A critical node in this network is the posterior cingulate, precuneal, and retrosplenial cortices. The cytological and neurochemical composition of this region is reviewed in relation to the Brodmann map. This region has the highest level of cortical glucose metabolism and cytochrome c oxidase activity. Monkey studies suggest that the anterior thalamic projection likely (...) drives retrosplenial and posterior cingulate cortex metabolism and that the midbrain projection to the anteroventral thalamic nucleus is a key coupling site between the brainstem system for arousal and cortical systems for cognitive processing and awareness. The pivotal role of the posterior cingulate, precuneal, and retrosplenial cortices in consciousness is demonstrated with posterior cingulate epilepsy cases, midcingulate lesions that de-afferent this region and are associated with unilateral sensory neglect, observations from stroke and vegetative state patients, alterations in blood flow during sleep, and the actions of general anesthetics. Since this region is critically involved in self reflection, it is not surprising that it is similarly a site for the NNCC. Interestingly, information processing during complex cognitive tasks and during aversive sensations such as pain induces efforts to terminate self reflection and result in decreased processing in posterior cingulate and precuneal cortices. (shrink)

Philosophers have proposed various kinds of relations between Mendelian genetics and molecular biology: reduction, replacement, explanatory extension. This paper argues that the two fields are best characterized as investigating different, serially integrated, hereditary mechanisms. The mechanisms operate at different times and contain different working entities. The working entities of the mechanisms of Mendelian heredity are chromosomes, whose movements serve to segregate alleles and independently assort genes in different linkage groups. The working entities of numerous mechanisms of molecular biology are larger (...) and smaller segments of DNA plus related molecules. Discovery of molecular DNA mechanisms filled black boxes that were noted, but unilluminated, by Mendelian genetics. (shrink)

Durante los años 1880, Nietzsche propone una idea original de individuo en tanto pluralidad como un modo de superación del difundido nihilismo con respecto a la unidad. Sus escritos, tanto editados como inéditos, presentan una crítica aguda de la concepción heredada de unidad anímica y cultural acorde con la crisis de identidad que vive Europa en la segunda mitad del siglo xix. Nuestro objetivo es mostrar un hilo que va desde la biología celular hasta la filosofía de Nietzsche. Consideramos cómo (...) la biología celular, especialmente con Rudolph Virchow, inauguró la crítica de la noción de Individuum desde el plano orgánico. Eso constituyó un motivo fuerte en la psicología de los franceses Hippolyte Taine y Theódule Ribot, quienes transpusieron el problema al ámbito anímico y de la psicopatología. Ambos concluyeron con un fuerte cuestionamiento sobre la unidad y simplicidad del yo. Eso generó un profundo interés en la filosofía de Nietzsche que en repetidas ocasiones se refiere al Individuum como una multiplicidad de células. Finalmente, profundizaremos en el registro nietzscheano acerca del Individuum, ya que constituye uno de los costados del pensamiento sobre el Übermensch, que Nietzsche imagina inspirado en los grandes hombres del Renacimiento, como posible superación del nihilismo. Among possible ways that Nietzsche can take to deal with Nihilism and its challenges, he proposes an original idea of plural individual. His papers both published and unpublished, present a sharp critique of the inherited view of psychic and cultural unit. It was in line with the identity's crisis faced by Europe along the second half of the nineteenth century. Our goal is to show a thread that goes from cell biology to Nietzsche's philosophy. We see how cell biology, precisely through Rudolph Virchow, inaugurated the critique of the notion of organic Individuum from the organic field. It became a strong subject in the psychology of Hippolyte Taine and Theódule Ribot. They transposed the issue to the field of mind and psychopathology. Both concluded in a strong challenge on the self's unity and simplicity. This generated a deep concern in Nietzsche's philosophy. He repeatedly refers to Individuum as a multiplicity of cells. Finally, we will go in deep into the Nietzschean consideration about Individuum because this topic means one important aspect of the concept of Übermensch that Nietzsche imagines inspired by the great men of the Renaissance as a promising way out of Nihilism. (shrink)

The Papanicolaou (Pap) smear, also called the Pap test, cyto test, cervical smear or cervical cytology, has been described as the most widely used and established cancer-screening technology in the world. It has also been described as a very simple technology including a brush, a microscope slide, fi xative and cervical cells from women. In 1928, George N. Papanicolaou, a Medical Doctor, investigator, PhD in zoology and Aureli Babes (1928/1967), a Romanian pathologist, each independently claimed to have found a ‘very (...) simple’ technique, which provided a new possibility for early diagnostics of cancer/malignant tumours in the female genital tract/uterine cervix. The technique was subsequently named after Papanicolaou who, according to Wied (1964: 174), was more successful than Babes in ‘stimulating the introduction of mass screening projects which are the actual benefi t of the method’. (shrink)

David Waldron Smithers was, among other things, a physician and a pioneer of cancer radiotherapy and a well-respected figure in British medicine and public health. From the 1940s until his retirement from medical practice in 1973, he was the director of the Radiotherapy Department at the Royal Marsden Hospital and London University Chair of Radiotherapy at the Institute of Cancer Research. Using massive amounts of clinical observations, which he interpreted from an organicist viewpoint, and his impressive synthetic thinking, he proposed (...) a coherent alternative perspective to the somatic mutation theory (SMT) which was then, and still is, the dominant theory of cancer. The purpose of this essay is to acquaint the modern audience with his seminal paper, published in 1962, because it deserves to be recognized as a true classic. In it, he examined the lack of fit between clinical observations and the SMT and proposed the rejection of this reductionist perspective. In addition, he built an organicist alternative in which carcinogenesis is seen as a problem of biological organization. His conceptual contribution to the cancer problem has inspired us and other authors over the last two decades. His essay “Cancer: An Attack on Cytologism,” originally published in The Lancet in 1962, is available as supplementary material in the online version of this article. (shrink)

This article considers the reception of British cytogeneticist C.D. Darlington's controversial 1932 book, Recent Advances in Cytology. Darlington's cytogenetic work, and the manner in which he made it relevant to evolutionary biology, marked an abrupt shift in the status and role of cytology in the life sciences. By focusing on Darlington's scientific method -- a stark departure from anti-theoretical, empirical reasoning to a theoretical and speculative approach based on deduction from genetic first principles -- the article characterises the relationships defining (...) the "disciplinary landscape" of the life sciences of the time, namely those between cytology, genetics, and evolutionary theory. (shrink)

Modern health care is inextricably bound up with technologically mediated knowledge and practice. It is vital to investigate its use and role in different clinical contexts characterized, on one hand, by face to face practitioner and patient encounters (where technology may be conceptualised as hindering therapeutic relations) and, on the other hand, by practitioners’ encounter with bodily parts in laboratories (where conceiving of patients may be thought of as confounding objectivity). To contribute to the latter, I offer an ethnographic analysis (...) of cytology laboratory practitioners’ work and microscopic assessment of normal and abnormal cells. First, I discuss the biomedical literature on cytology and the quest for a non-variational bodiless vision. Second, I discuss the concept of multistability, first developed by philosopher of technology Don Ihde, here used to analyse technologically mediated perception and how practitioners interact with technology. Combined with long term ethnographic fieldwork it enables access to, and analysis and articulation of the implicit multifaceted practitioner–technology–cell interface embedded in clinical practice and diagnostic processes. I will also address some implications of my analysis for clinical cytology. (shrink)

It has come to my attention that a number of formulations in the section “Disciplinary Landscape: Cytology and Genetics” of my article “Method as a Function of Disciplinary Landscape: C.D. Darlington and the History of Cytology 1925–1950,” Journal of the History of Biology, 39, 2006, pp. 165–197, do not provide due credit to a source. While Franz Schrader, “Three Quarter Centuries of Cytology,” Science 107 : 155–159, is cited in the article, his reminiscences and analysis of the historical development of (...) cytology provided a helpful historiographical context, as well as specific insights, for my article and should have been more fully acknowledged. (shrink)

The marriage of Sally Peris Hughes (1895–1984) and Franz Schrader (1891–1962) in November 1920 launched a highly successful scientific collaboration that lasted over four decades. The Schraders were avid naturalists, adroit experimentalists, and keen theoreticians, and both had long, productive, and fruitful careers in zoology. They offer an extraordinarily rich case study that provides an insightful view of the work carried out in several areas of the life sciences from the 1920s to the 1960s—fieldwork, cytology, cytogenetics, and entomology—as well as (...) critical aspects of the social world of contemporary science. By focusing on the fieldwork the couple carried out in Mexico and Central America in the late 1920s and early 1930s, this paper seeks to illuminate how this collaborative scientific marriage embodies a collective, complex, and integrated personal and social arrangement that served to enhance both knowledge production and disciplinary development in several areas of science. It also reveals ways in which marriage could serve as a means to help both parties navigate and negotiate restrictive sociocultural norms and institutional arrangements in science involving gender, power, and authority in the early twentieth century. (shrink)

In the period 1875-1920, a debate about the generality and applicability of evolutionary theory to all organisms was motivated by work on unicellular ciliates like Paramecium because of their peculiar nuclear dualism and life cycles. The French cytologist Emile Maupas and the German zoologist August Weismann argued in the 1880s about the evolutionary origins and functions of sex (which in the ciliates is not linked to reproduction), and death (which appeared to be the inevitable fate of lineages denied sexual conjugation), (...) an argument rooted in the question of whether the ciliates and their processes where homologous to other cellular organisms. In the beginning of the twentieth century, this question of homology came to be less important as the ciliates were used by the British protozoologist Clifford Dobell and the American zoologist Herbert Spencer Jennings to study evolutionary processes in general rather than problems of development and cytology. For them, homology mattered less than analogy. This story illustrates two partially distinct problems in evolutionary biology: first, the question of whether all living things have common features and origins; and second, whether their history and current nature can be described by identical mechanisms. Where Maupas (contra Weismann) made the ciliates qualitatively the same as all other organisms in order to create a cohesive evolutionary theory for biology, Jennings and Dobell made them qualitatively different in order to achieve the same end. (shrink)

Diagrams make it possible to present scientific facts in more abstract and generalized form. While some detail is lost, simplified and accessible knowledge is gained. E. B. Wilson's work in cytology provides a case study of changing uses of diagrams and accompanying abstraction. In his early work, Wilson presented his data in photographs, which he saw as coming closest to “fact.” As he gained confidence in his interpretations, and as he sought to provide a generalized textbook account of cell development, (...) he relied on increasingly abstract diagrams. In addition, he came to see that highly abstract and even schematic drawings could provide more than pictures directly from life. (shrink)

T. H. Morgan, A. H. Sturtevant, H. J. Muller and C. B. Bridges published their comprehensive treatise "The Mechanism of Mendelian Heredity" in 1915. By 1920 Morgan 's "Chromosome Theory of Heredity" was generally accepted by geneticists in the United States, and by British geneticists by 1925. By 1930 it had been incorporated into most general biology, botany, and zoology textbooks as established knowledge. In this paper, I examine the reasons why it was accepted as part of a series of (...) comparative studies of theory-acceptance in the sciences. In this context it is of interest to look at the persuasiveness of confirmed novel predictions, a factor often regarded by philosophers of science as the most important way to justify a theory. Here it turns out to play a role in the decision of some geneticists to accept the theory, but is generally less important than the CTH's ability to explain Mendelian inheritance, sex-linked inheritance, non-disjunction, and the connection between linkage groups and the number of chromosome pairs; in other words, to establish a firm connection between genetics and cytology. It is remarkable that geneticists were willing to accept the CTH as applicable to all organisms at a time when it had been confirmed only for Drosophila. The construction of maps showing the location on the chromosomes of genes for specific characters was especially convincing for non-geneticists. (shrink)

In 1853, the young Thomas Henry Huxley published a long review of German cell theory in which he roundly criticized the basic tenets of the Schleiden-Schwann model of the cell. Although historians of cytology have dismissed Huxley's criticism as based on an erroneous interpretation of cell physiology, the review is better understood as a contribution to embryology. "The Cell-theory" presents Huxley's "epigenetic" interpretation of histological organization emerging from changes in the protoplasm to replace the "preformationist" cell theory of Schleiden and (...) Schwann (as modified by Albert von Kölliker), which posited the nucleus as the seat of organic vitality. Huxley's views influenced a number of British biologists, who continued to oppose German cell theory well into the twentieth century. Yet Huxley was pivotal in introducing the new German program of "scientific zoology" to Britain in the early 1850s, championing its empiricist methodology as a means to enact broad disciplinary and institutional reforms in British natural history. (shrink)

From cytological examination, the size and form of the chromosomes in the eukaryotic nucleus are invariant across generations, leading to the expectation that constancy of inheritance likely depends on constancy of the chromosomal DNA molecule conveying the constant phenotype. Indeed, except for rare mutations, major phenotypic traits appear largely without change from generation to generation. Thus, when it was discovered that the inheritance of traits for bacteria, mitochondria and chloroplasts was also constant, it was assumed that chromosomes in those locations (...) were also constant. Such has not turned out to be the case, however; those chromosomes are highly variable in structure. I propose, therefore, that only for the nucleus is there a requirement that a chromosome be “finished” (contain only fully replicated genomes) before it may segregate to daughter cells. This requirement does not apply to the variable chromosomes among chloroplasts, mitochondria and bacteria. BioEssays 29:474–483, 2007. © 2007 Wiley Periodicals, Inc. (shrink)

In Search Of Mechanisms is a book about the methodology of biology. It is a work by Carl Craver and Lindley Darden, both of whom are well-known individually for their advocacy of mechanistic explanation—in the neurosciences and in the fields of genetics, cytology and molecular biology . Here, the two join forces to give a unified model of biological explanation, not limited to a particular area of biological enquiry, as rooted in the search for mechanisms.The objectives of the book are (...) threefold. First, it sets out to describe what biologists do. This first objective can be described as the goal of making biology more comprehensible. If biology involves the search for mechanisms, then one cannot understand the history of biology and its current practice without understanding mechanisms. Secondly, the book seeks to give a proscriptive account of explanation and the process of explaining some phenomena. This second objective relates to an interest in contributing to the .. (shrink)

Predictions about the health risks of low level radiation combine two sorts of measures. One estimates the amount and kinds of radiation released into the environment, and the other estimates the adverse health effects. A new field called health physics integrates and applies nuclear physics to cytology to supply both these estimates. It does so by first determining the kinds of effects different types of radiation produce in biological organisms, and second, by monitoring the extent of these effects produced by (...) given levels of exposure. This essay examines the interplay between evidential constraints and external, contextual interests and values in studying the biological hazards of radiation. By analyzing the debate over linear vs. quadratic dose response models, the essay focuses on problems of developing quantitative, rather than qualitative, estimates of the risks of increased cancer incidence in an exposed population. (shrink)

In this paper, I wish to challenge theory-biased approaches to scientific knowledge, by arguing for a study of theorizing, as a cognitive activity, rather than of theories, as abstract structures independent from the agents’ understanding of them. Such a study implies taking into account scientists’ reasoning processes, and their representational practices. Here, I analyze the representational practices of geneticists in the 1910s, as a means of shedding light on the content of classical genetics. Most philosophical accounts of classical genetics fail (...) to distinguish between the purely genetic, or Mendelian level, and the cytological one. I distinguish between them by characterizing them in terms of their respective associated representational practices. I then present how the two levels were articulated within Morgan’s theory of crossing-over, and I describe the representational technique of linkage mapping, which embodies the “merging” of the Mendelian and cytological levels. I propose an analysis of the mapping scheme, as a means of enlightening the conceptual articulation of Mendelian and cytological hypotheses within classical genetics. Finally, I present the respective views of three opponents to Morgan in the 1910s, who had a different understanding of the articulation of cytology and Mendelism, and entertained different views concerning the role and proper interpretation of maps. I propose to consider these diverging perspectives as instantiating what I call different “versions” of classical genetics. (shrink)

One claim found in the received historiography of the biometrical school (comprised primarily of Francis Galton, Karl Pearson, and W. F. R. Weldon) is that one of the biometricians' great flaws was their inability to look past their population-focused, statistical, gradualist understanding of evolutionary change – which led, in part, to their ignoring developments in cellular biology around 1900. I will argue, on the contrary, that the work of the biometricians was, from its earliest days, fundamentally concerned with connections between (...) statistical patterns of inheritance and the underlying cellular features that gave rise to them. Such work remained current with contemporary knowledge of chromosomes, cytology, and development; in this article, I explore the first case. The biometricians were thus well positioned to understand the relationship between the patterns of Mendelian inheritance and the statistical distributions with which they primarily occupied themselves. Ignorance of this connection, then, is not the reason why they rejected Mendelism. Further, both Galton and Weldon – though each in their own unique way – decided to turn to biological detail as a way to better justify the generality of their statistical approaches to heredity. Perhaps paradoxically, then, for these biometricians, detail offered an approach to theoretical generality. (shrink)

This paper gives a detailed narrative of a controversial empirical research in postwar population genetics, the analysis of the cytological polymorphisms of an Australian grasshopper, Moraba scurra. This research intertwined key technical developments in three research areas during the 1950s and 1960s: it involved Dobzhansky’s empirical research program on cytological polymorphisms, the mathematical theory of natural selection in two-locus systems, and the building of reliable estimates of natural selection in the wild. In the mid-1950s the cytologist Michael White discovered an (...) interesting case of epistasis in populations of Moraba scurra. These observations received a wide diffusion when theoretical population geneticist Richard Lewontin represented White’s data on adaptive topographies. These topographies connected the information on the genetic structure of these grasshopper populations with the formal framework of theoretical population genetics. As such, they appeared at the time as the most successful application of two-locus models of natural selection to an empirical study system. However, this connection generated paradoxical results: in the landscapes, all grasshopper populations were located on a ridge while they were expected to reach a peak. This puzzling result fueled years of research and triggered a controversy attracting contributors from Australia, the United States and the United Kingdom. While the original problem seemed, at first, purely empirical, the subsequent controversy affected the main mathematical tools used in the study of two-gene systems under natural selection. Adaptive topographies and their underlying mathematical structure, Wright’s mean fitness equations, were submitted to close scrutiny. Suspicion eventually shifted to the statistical machinery used in data analysis, reflecting the crucial role of statistical inference in applied population genetics. In the 1950s and 1960s, population geneticists were not simply in search for new generalizations about the evolutionary process and for new evidence about genetic variation: struggling against statistical artifacts, they were searching for reliable tests and descriptors to analyze their data. (shrink)

Introduction A Brief Conceptual Framework for Biology PART ONE: UNDERSTANDING NATURE 1. The Antecedents of Scientific Thought Animism, Totemism, and Shamanism The Paleolithic View Mesopotamia Egypt 2. Aristotle and the Greek View of Nature The Science of Animal Biology The Parts of Animals The Classification of Animals The Aristotelian System Basic Questions 3. Those Rational Greeks? Theophrastus and the Science of Botany The Roman Pliny Hippocrates, the Father of Medicine Erasistratus Galen of Pergamum The Greek Miracle 4. The Judeo-Christian Worldview (...) The Bishop of Hippo Scholastic Thought Islamic Science Books on Beasts Antecedents of a Revolution 5. The Revival of Science Andreas Vesalius and the Study of Structure William Harvey and the Study of Function Sir Francis Bacon’s Great Instauration Induction, Hypothesis, Deduction The Very Small--Animalcules Robert Hooke and the Discovery of Cells 6. Figur’d Stones and Plastick Virtue Marine Life on Mountain Tops? Figured Stones of Unknown Creatures Baron Cuvier Quarries of the Paris Basin Catastrophism and Uniformitarianism William Smith and the Geological Column Understanding Nature in 1850 PART TWO: THE GROWTH OF EVOLUTIONARY THOUGHT 7. The Paradigm of Evolution First Questions The Paradigm of Natural Theology First Answers 8. Testing Darwins Hypotheses Have Life Forms Changed over Time? Do Species Evolve into Different Species over Time? Has There Been Time Enough for Evolution? Is Natural Selection the Mechanism of Change? The Genetic Basis of Natural Selection Accounting for the Diversity of Life 9. In the Light of Evolution Comparative Anatomy Embryonic Development Classification Microstructure Molecular Processes 10. Life over Time The Origin of Life The Rise of Multicelled Organisms What Is a Phylum? Burgess Shale Metazoans Early Evolution of the Vertebrates The Age of Dinosaurs Birds, Mammals, and Flowering Plants The Rancho La Brea Tar Pits Human Evolution The Role of Extinction in Evolution PART THREE: CLASSICAL GENETICS 11. Pangenesis What Is the Question? Hippocrates and Aristotle The Darwinian Answer Assembling the Data Formulating the Hypothesis by Induction Galton’s Rabbits 12. The Cell Theory The Discovery of Cells: Robert Hooke Schwann and Cells in Animals Gametes as Cells Omnis cellula e cellula? The Technology of Cell Research 13. The Hypothesis of Chromosomal Continuity The Ephemeral Nucleus Schneider, Flemming, and Cell Division The Chromosomes and inheritance Gamete Formation Fertilization 14. Mendel and the Birth of Genetics Model for Monohybrid Crosses Model for Dihybrid Crosses Mendel’s Laws Initial Opposition to Mendelism 15. Genetics + Cytology: 1900-1910 Sutton’s Model The Cytological Basis of Mendel’s Laws Boveri and Abnormal Chromosome Sets Variations in Mendelian Ratios The Discovery of Sex Chromosomes 16. The Genetics of the Fruit Fly Morgan’s First Hypothesis Morgan’s Second Hypothesis The Fly Room Linkage and Crossing-Over The Cytological Proof of Crossing-Over Mapping the Chromosomes The Final Proof The Determinants of Sex The Conceptual Foundations of Classical Genetics 17. The Structure and Function of Genes One Gene, One Enzyme The Substance of Inheritance The Watson-Crick Model of DNA Genes and the Synthesis of Proteins The Genetic Code PART FOUR: THE ENIGMA OF DEVELOPMENT 18. First Principles The Peripatetic Stagirite The Death and Rebirth of Scientific Thought Harvey and Malpighi A Two-Millennial Summing Up Preformation versus Epigenesis 19. The Century of Discovery Von Baer’s Discovery of the Mammalian Ovum Darwin’s Contribution to Embryology Haeckel and Recapitulation 20. Descriptive Embryology Germ Layers The External Development of the Amphibian Embryo The Internal Development of the Amphibian Embryo 21. The Dawn of Analytical Embryology His, Roux, and Mosaic Development Driesch and Regulative Development Novelty in Development Cell Lineage Nucleus or Cytoplasm? Fin de Siècle 22. Interactions during Development Amphibian Organizers Secondary Organizers The Reacting Tissue The Chemical Nature of the Organizer Putting It All Together Conclusion Further Reading References Illustration Credits Index. (shrink)

This paper analyzes features of the emergence of new fields in science by examining the cases of cytology and biochemistry. The first step in the emergence of these new fields was the discovery of a new entity. A subsequent claim was made that entities of this kind are found more generally; making this generalization constituted the construction of a new theory. As a line of research to test the theory began, a new domain was formed and the new field emerged. (...) In each case the theory proposed a new solution to an old problem in science. Some implications of this analysis for understanding the emergence of other fields are indicated. (shrink)

Genesis: The Evolution of Biology presents a history of the past two centuries of biology, suitable for use in courses, but of interest more broadly to evolutionary biologists, geneticists, and biomedical scientists, as well as general readers interested in the history of science. The book covers the early evolutionary biologists-Lamarck, Cuvier, Darwin and Wallace through Mayr and the neodarwinian synthesis, in much the same way as other histories of evolution have done, bringing in also the social implications, the struggles with (...) our religious understanding, and the interweaving of genetics into evolutionary theory. What is novel about Sapp's account is a real integration of the cytological tradition, from Schwann, Boveri, and the other early cell biologists and embryologists, and the coverage of symbiosis, microbial evolutionary phylogenies, and the new understanding of the diversification of life coming from comparative analyses of complete microbial genomes. The book is a history of theories about evolution, genes and organisms from Lamarck and Darwin to the present day. This is the first book on the general history of evolutionary biology to include the history of research and theories about symbiosis in evolution, and first to include research on microbial evolution which were excluded from the classical neo-Darwinian synthesis. Bacterial evolution, and symbiosis in evolution are also excluded from virtually every book on the history of biology. (shrink)

In the space of three years–from 1962 to 1964 – the proposition that chloroplasts contain their own DNA made the transition from being a controversial hypothesis to an accepted dogma. The crucial evidence came from biochemical analyses of the organelles themselves and from cytological studies. These discoveries revolutionized our views on the distribution of genetic information within the cell, and gave rise to the vigorous new field of chloroplast molecular biology. It is nevertheless ironic to recall that of the biochemical (...) papers published on this topic in 1962–64, the one which was probably the most influential in creating the new paradigm was in fact quite wrong, and attributed to the chloroplast, DNA species which did not in reality belong to this organelle at all. (shrink)

In historical literature, Edouard van Beneden is mostly remembered for his cytological discoveries. Less well known, however, is that he also introduced evolutionary morphology – and indeed evolutionary theory as such – in the Belgian academic world. The introduction of this research programme cannot be understood without taking both the international and the national context into account. It was clearly the German example of the Jena University that inspired van Beneden in his research interests. The actual launch of evolutionary morphology (...) at his University of Liège was, however, also connected with the dynamic of Belgian university reforms and the local rationale of creating a research “school.” Thanks to his networks, his mastering of the rhetoric of the “new” biology, his low ideological profile and his capitalising on the new academic élan in late-19th century Belgium, van Beneden managed to turn his programme into a local success from the 1870s onwards. Two decades later, however, the conceptual underpinnings of evolutionary morphology came under attack and the “Van Beneden School” lost much of its vitality. Despite this, van Beneden’s evolutionary morphology was prototypical for the research that was to come. He was one of the first scientific heavyweights in Belgium to turn the university laboratory into a centre of scientific practice and the hub of a research school. (shrink)

By inferences from fossil records and circumstantial evidences, it is now generally postulated that angiosperms have a much longer history than hitherto believed and that they have already existed probably in Jurassic time. Studies in vascular tissues and reproductive, structures have negated the possibility of originating angiosperms from various gymnosperm groups. Chronologically, this derivation will be also an impossibility.From a consideration of various aspects in the life history of angiosperms, a hypothesis is here presented postulating that protangiosperms originated in an (...) aquatic or subaquatic environment from an algal ancestry. The enclosure of ovules in the ovary is interpretated as a means of protection necessitated for the transmigration from an aquatic to an aerial habitat for the developing reproductive parts in the bud underwater. Trimery is suggested as a primitive condition, one that affords maximum efficiency in carrying out the purpose under the conditions.In the life history of angiosperms, the endosperm is of great significance in phylogeny. From morphological, physiological, cytological as well as genetical considerations, the endosperm should be regarded as of equal status with the gametophytic and sporophytic generations. There is no comparable structure in the gymnosperm groups and the ferns and “fern-allies”, while more or less similar conditions are present in the thallophytes. In the long life histories of many red algae, however, there is an especially notable resemblance to angiosperms, with certain details approaching the unique and characteristic features of the endosperms.Land plants are presumably all evolved from aquatic ancestors but instead of the usual concept of a monophyletic origin and lineal development of all vascular plants, a more plausible suggestion is the multiple and recurrent derivation of such plants from various algal ancestral groups. The angiosperms probably represent one of such major lines that originated more or less directly from a thallophytic ancestry. (shrink)

The article aims at providing grounds for the analysis of the confrontation of scientific views in the context of E. Agassi’s famous thesis: “science is always socially engaged, but it should not be socially dependent”. This socio-epistemological analysis focuses on the clash between Lysenko’s interpretation of the Lamarckian methodology of H. Spencer’s views and representatives of the Russian school of classical Mendelian genetics. A distinctive feature of Akademgorodok in the 1970s-1980s was that almost every academic institute had a person responsible (...) for organizing a specialized scientific-methodological seminar on philosophical and methodological problems of a particular field of knowledge. Recollections of the participants of these seminars and reconstruction of the issues discussed at that time are rich empirical material, providing an opportunity to test modern conceptions in the field of social anthropology, social epistemology, and sociology of science. The revival of genetics in our country began with the founding of the Institute of Cytology and Genetics of the Siberian Branch of the USSR Academy of Sciences/SB RAS. It is shown that this confrontation between Lamarckism and Mendelism in 1930s-1960s develops from the denial of the theses of the ideological opponents to the emergence of accompanying interdisciplinary research. At the same time, a special role is given to the specification of the methodological function of philosophy and to those forms of its realization that scientists personally participating in scientific-methodological seminars, were more inclined to associate with “substantive” rather than “sociological” aspects of the description of the situation. (shrink)

The origin of culture remains in the sphere of hypotheses. Although the hypotheses derive from two presumptions: first, how the structure of culture is envisaged, and secondly, how culture is thought to function. Juri Lotman dealt with both aspects of culture, initially the structural and typological and later the dynamic aspects. Thereby, he arrived at the culturalphilosophical hypothesis of the autocatalytic origin of culture. A catalyst is a component of a chemical reaction which itself doesn’t transform during the reaction, but (...) whose presence is needed to guarantee the reaction (or to stimulate it). Thus, autocatalysis is a paradoxical situation in which the genesis of something presumes the pre-existence of the final product. The paradox of the autocatalysis of culture lies in the fact that culture cannot emerge from anything other than from culture itself, from its own germination. In 1988, speaking about the autocatalysis of culture, Lotman refered tothe cultural historicist Nikolai I. Konrad (1891–1970), who undoubtedly borrowed this idea from Jacob Christopher Burckhardt (1818–1897). This undiscovered connection reminds us of the fact, that a model for autocatalysis (or an autopoiesis) was basic to Naturphilosophie of the 19th century. In the 20th century, this was represented by Vladimir I. Vernadsky (1863–1945), from whom Lotman in 1982 received the impetus to formulate the concept of semiosphere as well as of the autocatalysis of culture. The autocatalysis model of culture is culturally diachronical, the semiosphere is, however, a synchronical one. In both cases, the natural philosophical cytology of the 19th century was Lotman’s semiotical meta-language. (shrink)

Meiotin‐1 is a protein found in developing microsporocytes of Lilium longiflorum, and immunological assays indicate that cognates exist in both mono‐ and dicotyledonous plants. Its temporal and spatial expression pattern, coupled with its unusual distribution in chromatin and the properties it shares with histone H1, encourages speculation that it is involved in regulating meiotic chromatin structure. Molecular analyses provide support for the hypothesis that meiotin‐1 arose from histone H1 by an exon shuffling mechanism, as meiotin‐1 is an H1‐like protein that (...) lacks the amino‐terminal domain shared by H1 molecules. We have proposed that meiotin‐1 serves to limit chromatin condensation in order to foster the unique cytological and molecular events which occur during meiotic prophase. As such, meiotin‐1 fits the role of a ‘meiosis readiness factor’, and its accumulation to a threshold level may commit mitotically dividing progenitor cells to differentiate into meiocytes. (shrink)

It is argued that the assessment of the strength of the evidence for the Darwinian theory of evolution by natural selection offered by Michael Ruse in the Philosophy of Biology is in one respect too weak and in the other too strong. His claim that artificial selection provides at best analogical evidence for the theory is shown to rest on a spurious distinction between artificial and natural selection. His argument that Darwinian theory, unlike its competitors, accounts for the cytological and (...) genetic data is demonstrated to be unwarranted and fails to differentiate the actual degrees of evidential support provided by cytology and genetics for parts of differing and competing theories of evolution. The evidentially secure foundations of Darwin's theory are not challenged in this paper, only Ruse's account of their nature. (shrink)

What are cells? How are they related to each other and to the organism as a whole? These questions have exercised biology since Schleiden and Schwann (1838–1839) first proposed cells as the key units of structure and function of all living things. But how do we try to understand them? Through new technologies like the achromatic microscope and the electron microscope. But just as importantly, through the metaphors our culture has made available to biologists in different periods and places. These (...) two new volumes provide interesting history and philosophy of the development of cell biology. Reynolds surveys the field's changing conceptual structure by examining the varied panoply of changing metaphors used to conceptualize and explain cells – from cells as empty boxes, as building blocks, to individual organisms, to chemical factories, and through many succeeding metaphors up to one with great currency today: cells as social creatures in communication with others in their community. There is some of this approach in theVisionsedited collection as well. But this collection also includes rich material on the technologies used to visualize cells and their dialectical relationship with the epistemology of the emerging distinct discipline of cell biology. This volume centres on, but is not limited to, ‘reflections inspired by [E.V.] Cowdry's [1924 volume]General Cytology’; it benefits from a conference on the Cowdry volume as well as a 2011 Marine Biological Lab/Arizona State University workshop on the history of cell biology. (shrink)

Oogenesis being performed in the ovary shows two different kinds of nuclei in the nursing cells. The above mentioned nuclei are transferred and incorporated into the nuclei of developing eggs, which become sexually differentiated and showWolanski's methyl-green reaction. The sex determination is, therefore, cytologically progamic and genotypical. The spawned eggs in the jelly strings appear first of identical shape and are all coated from the very beginning with grainy bonellian pigment, but afterwards, being reared in free water cultures in the (...) absence of females, they differentiate into distinctly different male and female larvae. The numerical ratio of the sexually differentiated larvae is discussed. The true chromosomes in gamogenesis as well as in somatic cells have not been found. L'oogénèse qui a lieu dans l'ovaire montre deux sortes de noyaux dans les cellules nourricières. Les noyaux mentionnés ci-dessus sont transportés dans les noyaux des ovules en voie de développement qui se différencient sexuellement et montrent la réaction deWolanski au vert de méthyle. Le déterminisme du sexe est, par conséquent progamique et génotypique, du point de vue cytologique. Les ovules déposés en gelée sont d'abord de forme identique et sont tous recouverts, dès le début, du pigment granulé de boneliéne, mais, élevés par la suite, dans des cultures en eau courante, en l'absence des femelles, ils se différencient distinctement en larves mâles et femelles. La proportion numérique des larves différenciées sexuellement est discutée. Dans la gamétogénèse, comme dans les cellules somatiques, les vrais chromosomes restent introuvables. Während der Oogenese, die sich im Eierstock abspielt, zeigen sich zwei differente Kerntypen in den Nährzellen. Diese Kerne werden allmählich in die Kerne des sich entwickelnden Eies übertragen und dort inkorporiert. Das Ei wird dadurch geschlechtlich differenziert und zeigt dann dieWolanski's Methyl-grün Reaktion. Die sexuelle Determination ist somit progam und genotypic. Die in schnurartigen Gallerten abgelegten Eier sind am Beginn von gleicher Form und Grösse, so wie von Anfang an mit Körnchen des Pigmentes Bonellein bekleidet. In freien Wasserkulturen,ohne Weibchen, entwickeln sie sich in deutlich differenzierte männliche und weibliche Larven. Das numerische Verhältniss der geschlechtlich differenten Larven wird diskutiert. Echte Chromosomen sind weder bei der Gamogenese noch in somatischen Zellen gefunden worden. (shrink)

Bateson's belated acceptance of the chromosome theory came in two main stages, and was permanent, although he retained to the end reservations about some implications and extensions of the theory. Coleman's attempt to explain Bateson's resistance in terms of his conservative mode of thought is critically examined, and rejected: the attributes Coleman assigns to Bateson are all either inappropriate, or irrelevant to chromosome theory, or both. Instead, the diverse factors which contributed to Bateson's resistance are enumerated and discussed. These include (...) deficiencies in the evidence then available to support the theory; embryological difficulties; Bateson's aversion to microscopy combined with the lack of a close colleague skilled in cytology; his iconoclastic temperament, and the fact that he found T. H. Morgan, the leader of the Drosophila school, an uncongenial personality. Taken together, these factors suffice to account for his resistance. Bateson's position is contrasted with that of the other most eminent opponent, W. Johannsen, whose organismic outlook was the main obstacle to acceptance. (shrink)

Biologists employ a suggestive metaphor to describe the complexities of molecular interactions within cells and embryos: cytological components are said to be part of “ecosystems” that integrate them in a complex network of relations with many other entities. The aim of this essay is to scrutinize the molecular ecosystem, a metaphor that, despite its longstanding history, has seldom be articulated in detail. I begin by analyzing some relevant analogies between the cellular environment and the biosphere. Next, I discuss the applicability (...) of the molecular ecosystem concept in actual scientific practice. (shrink)

Early in the 20th century Bateson, Doncaster and Punnett formed a cooperative collective to share research findings on the chromosome theory of heredity (CTH). They cross-bred plants and animals to correlate behaviour of chromosomes and heredity of individual traits. Doncaster was the most enthusiastic proponent of the new theory and worked for years to convince Bateson and Punnett on its relevance to their own research. The two younger biologists collaborated with Bateson, the preeminent geneticist in England. As their own reputations (...) developed, their research findings allied with the consensus on the importance of the CTH by the broader scientific community. After Doncaster’s tragic death in 1920, major objections to the theory had been resolved; Bateson and Punnett then utilized the CTH to construct chromosome maps detailing locations of specific genes on particular chromosomes in several different species. The marriage of heredity and cytology enhanced confidence that the theory was an accurate mechanism to explain inheritance in both plants and animals. (shrink)

Until recently the British zoologist Lancelot Hogben has usually appeared as a campaigning socialist, an anti-eugenicist or a popularizer of science in the literature. The focus has mainly been on Hogben after he became a professor of social biology at the London School of Economics in 1930. This paper focuses on Hogben’s life in the 1920s. Early in the decade, while based in London, he focused on cytology, but in 1922, after moving to Edinburgh, he turned his focus on experimental (...) zoology, first concentrating on vertebrate endocrinology and later moving over to the comparative physiology of invertebrate muscle. In the early 1920s Hogben played an active role in the development of experimental zoology in Britain. As such he was a fearless critic of evolutionary and metaphysical speculations. But in this period Hogben’s career prospects were seriously hampered by his confrontational nature and serious depression. As a result he was forced to leave Britain in 1925. He first accepted a position in Canada and in the period 1927–1930 he was a professor of zoology in South Africa. This paper will also add crucial new material to James Tabery’s recent discussion of the history behind Hogben’s ideas about the interaction of heredity and environment in individual development. In addition a previously unknown Lamarckian controversy will be discussed. (shrink)

This paper explores the eugenic thought of Yamanouchi Shigeo (1876–1973), who was trained in plant cytology under the tutelage of botanist and eugenicist John Coulter (1851–1928) in the USA, and later became one of the early and important popularizers of eugenic ideas in Japan. His career demonstrates a direct link between Japanese and US eugenics. Despite his academic training and research at various internationally renowned institutions, numerous publications, and longevity, his life has received little scholarly attention. By the early twentieth (...) century, most biologists in Japan, as in the USA, began accepting Mendelian evolutionary theory and rejecting the Lamarckian notion of inheritance of acquired characteristics. However, Yamanouchi Shigeo's eugenic view represents a paradox: he was a Mendelian cytologist sympathetic to Lamarckism. Was his ‘nurture’‐oriented eugenic view unscientific? Is that why he was largely ignored in the history of botany in Japan? This study attempts to answer these questions and to analyse the origins and distinct features of Yamanouchi's eugenic ideas by situating Yamanouchi's eugenic thought historically and culturally. After examining his scientific papers, popular writings, and documents of various organizations to which he belonged, I argue that Yamanouchi's ‘softer’ (or less biologically deterministic) perspective may have reflected the Japanese desire to catch up with the dominant ‘race’ by using eugenics without accepting permanent inferior status. (shrink)

This paper aims to illustrate the influence of Franz Unger’s (1800–1870) and Carl Wilhelm Nägeli’s (1817–1891) anatomical and developmental works on Gregor Mendel’s (1822–1884) use of numerical ratios in biological inquiry. All hypotheses concerning Mendel’s sources of inspiration have hitherto overlooked the cytological teaching of Unger, his professor of botany. In the 1830s and 1840s, he was a pioneer of cell theory. His publications, including his university textbooks, are characterised by a particulate and quantifying approach towards vital phenomena. Special attention (...) will be paid to Unger’s and Nägeli’s ‘law of plant growth’, which shows some striking basic correspondences with Mendel’s approach. This demonstrates, firstly, that mathematical thinking was not alien to mid-nineteenth century botany, and secondly, that Mendel’s conceptions were influenced by an even greater variety of ideas circulating around 1850. (shrink)