Genetic mapping of 66 new microsatellite loci in bread wheat

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Abstract

In hexaploid bread wheat, ten members of the IWMMN collaborated in extending the microsatellite genetic map. Among a much larger number of microsatellite primer pairs developed as a part of the WMC, 58 out of 176 primer pairs tested were found to be polymorphic between the parents of the ITMI mapping population W7984 x Opata 85. This population was used earlier for the construction of RFLP maps in bread wheat. Using the ITMIpop and a framework map prepared for this purpose, a total of 66 microsatellite loci were mapped, which were distributed on 20 of the 21 chromosomes. These 66 mapped microsatellite loci add to the existing 384 microsatellite loci earlier mapped in bread wheat.

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Brady Keller
Saint Mary's University of Minnesota
Russell Wang
Australian National University
Ruixin Wang
Ecole Nationale de l'Aviation Civile (ENAC)
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